Improvement of saccharide yield from wood by simultaneous enzymatic delignification and saccharification using a ligninolytic enzyme and cellulase

• Published in: Journal of bioscience and bioengineering Vol. 132; no. 3; pp. 213 - 219
• Main Authors: Mori, Toshio, Ikeda, Kohei, Kawagishi, Hirokazu, Hirai, Hirofumi
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-09-2021
• Subjects: Co-oxidants; In vitro degradation; Lignin; Manganese peroxidase; Phanerochaete sordida YK-624; Saccharification; Lignin; Saccharification; Phanerochaete sordida YK-624; In vitro degradation; Co-oxidants; Manganese peroxidase
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2021.04.016

White-rot fungi are thought to hold promise for development of a delignification pretreatment process for wood biorefinery that is less energy-consuming than current processes. However, the reaction must take place over weeks and consumes non-neglectable amounts of saccharides. To establish a biological process for wood biorefinery would first require establishment of an enzymatic approach to delignification. Such an approach has the potential to lower costs and reduce saccharide loss. Here, we attempted enzymatic delignification reactions using manganese peroxidases (MnP), a lignin-degrading enzyme, under several reaction conditions. The delignification rate from beech wood meal (particle size <45 μm) of up to 11.0% in 48 h was reached in a MnP reaction supplemented with multiple co-oxidants, glucose, glucose oxidase (GOD) and commercial cellulase. An additional 48-h reaction using fresh MnP/co-oxidants increased the delignification rate to 14.2%. Simultaneous enzymatic delignification and saccharification, which occurs without a need for glucose supplementation, successfully improved the glucose yield to 160% of the reaction without MnP. Development of a more accurate imitation of the mechanisms of delignification that occurs in white-rot fungi has the potential to improve the monosaccharide yield resulting from simultaneous delignification and saccharification.
•Simultaneous enzymatic delignification and saccharification (SDS) for wood was attempted.•Conditions of delignification using manganese peroxidase were investigated for SDS treatment.•Both delignification and saccharification activities seemed complemented each other.•The SDS improved glucose yield to 160% from wood biomass compared the treatment with cellulase alone.