Biofabrication of silver nanoparticles using bacteria from mangrove swamp

• Published in: IET nanobiotechnology Vol. 12; no. 5; pp. 626 - 632
• Main Authors: Sharma, Manish, Nayak, Parth Sarthi, Asthana, Shreyasi, Mahapatra, Dipankar, Arakha, Manoranjan, Jha, Suman
• Format: Journal Article
• Language: English
• Published: United States The Institution of Engineering and Technology 01-08-2018
• Subjects: Aeromonas veronii; Aeromonas veronii - isolation & purification; Aeromonas veronii - metabolism; amide‐I peaks; amide‐II peaks; Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; antibacterial activity; antibacterial potential; antimicrobial activity; attenuated total reflection; attenuated total reflection Fourier transform infrared spectroscopy; bacteria; bacteria‐mediated method; biochemistry; biological methods; biological system; biomedical materials; bioremediation; capping agents; chromatography; cost‐effective process; E. coli; eco‐friendly; field emission scanning electron microscopy; Fourier transform infrared spectra; mangrove swamp; metal ions; metal nanoparticle biofabrication; Metal Nanoparticles - chemistry; metal nanostructures; microorganisms; microstructures; molecular biophysics; molecular configurations; nanofabrication; nanomedicine; nanoparticles; nonconjugated biomolecules; particle size; proteins; purification; Research Article; S. aureus; silver; Silver - chemistry; Silver - pharmacology; silver nanoparticles; size 10 nm to 20 nm; size exclusion chromatography; stabilising agents; swamp wetland; TEM; transmission electron microscopy; ultraviolet spectra; UV‐visible spectroscopy; visible spectra; Water Microbiology; Wetlands; X‐ray diffraction; antibacterial activity; particle size; bioremediation; bacteria; X-ray diffraction; visible spectra; nanoparticles; capping agents; chromatography; attenuated total reflection Fourier transform infrared spectroscopy; transmission electron microscopy; nonconjugated biomolecules; UV-visible spectroscopy; size exclusion chromatography; metal ions; biomedical materials; silver; amide-II peaks; biological methods; molecular configurations; Fourier transform infrared spectra; metal nanostructures; Ag; nanofabrication; field emission scanning electron microscopy; Aeromonas veronii; S. aureus; purification; proteins; size 10 nm to 20 nm; attenuated total reflection; cost-effective process; E. coli; nanomedicine; antimicrobial activity; mangrove swamp; ultraviolet spectra; eco-friendly; biological system; silver nanoparticles; microorganisms; metal nanoparticle biofabrication; swamp wetland; biochemistry; amide-I peaks; antibacterial potential; bacteria-mediated method; microstructures; molecular biophysics; TEM; stabilising agents
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2017.0205

The last decade has observed a rapid advancement in utilising biological system towards bioremediation of metal ions in the form of respective metal nanostructures or microstructures. The process may also be adopted for respective metal nanoparticle biofabrication. Among different biological methods, bacteria-mediated method is gaining great attention for nanoparticle fabrication due to their eco-friendly and cost-effective process. In the present study, silver nanoparticle (AgNP) was synthesised via continuous biofabrication using Aeromonas veronii, isolated from swamp wetland of Sunderban, West Bengal, India. The biofabricated AgNP was further purified to remove non-conjugated biomolecules using size exclusion chromatography, and the purified AgNPs were characterised using UV–visible spectroscopy, X-ray diffraction, field emission scanning electron microscopy and transmission electron microscopy (TEM). Additionally, the presence of proteins as capping and stabilising agents was confirmed by the amide-I and amide-II peaks in the spectra obtained using attenuated total reflection Fourier transform infrared spectroscopy. The size of biofabricated AgNP was 10–20 nm, as observed using TEM. Additionally, biofabricated AgNP shows significant antibacterial potential against E. coli and S. aureus. Hence, biofabricated AgNP using Aeromonas veronii, which found resistant to a significant concentration of Ag ion, showed enhanced antimicrobial activity compared to commercially available AgNP.