A Catalogue of Molecular, Physiological and Symbiotic Properties of Soybean-Nodulating Rhizobial Strains from Different Soybean Cropping Areas of China

• Published in: Systematic and applied microbiology Vol. 26; no. 3; pp. 453 - 465
• Main Authors: Thomas-Oates, J., Bereszczak, J., Edwards, E., Gill, A., Noreen, S., Zhou, J.C., Chen, M.Z., Miao, L.H., Xie, F.L., Yang, J.K., Zhou, Q., Yang, S.S., Li, X.H., Wang, L., Spaink, H.P., Schlaman, H.R.M., Harteveld, M., Díaz, C.L., van Brussel, A.A.N., Camacho, M., Rodríguez-Navarro, D.N., Santamaría, C., Temprano, F., Acebes, J.M., Bellogín, R.A., Buendía-Clavería, A.M., Cubo, M.T., Espuny, M.R., Gil, A.M., Gutiérrez, R., Hidalgo, A., López-Baena, F.J., Madinabeitia, N., Medina, C., Ollero, F.J., Vinardell, J.M., Ruiz-Sainz, J.E.
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 01-09-2003; Elsevier; Elsevier Science Ltd
• Subjects: Agronomy. Soil science and plant productions; Bacterial Proteins - analysis; Biological and medical sciences; China; Congo Red - metabolism; DNA Fingerprinting; DNA, Bacterial - isolation & purification; DNA, Ribosomal - analysis; DNA, Ribosomal Spacer - analysis; Economic plant physiology; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; Glycine max - microbiology; Lipopolysaccharides - analysis; Melanins - biosynthesis; Nod factors; PCR analyses; plasmid profiles; Plasmids; Polymorphism, Restriction Fragment Length; Random Amplified Polymorphic DNA Technique; Rhizobium - chemistry; Rhizobium - genetics; Rhizobium - isolation & purification; Rhizobium - physiology; RNA, Ribosomal, 16S - genetics; Soybean-nodulating rhizobia; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); symbiotic characteristics; Asia; China; plasmid profiles; PCR analyses; Soybean-nodulating rhizobia; Nod factors; symbiotic characteristics; Symbiosis; Cultivated soil; Nodulation; Genotype; Rhizobium; Glycine max; Profile; Strain; Plasmid; Polymerase chain reaction; Phenotype; Leguminosae; Dicotyledones; Angiospermae; Nitrogen fixation; Bacteria; Spermatophyta; Rhizobiaceae
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1078/072320203322497491

We have analysed 198 fast-growing soybean-nodulating rhizobial strains from four different regions of China for the following characteristics: generation time; number of plasmids; lipopolysaccharide (LPS), nodulation factors (LCOs) and PCR profiles; acidification of growth medium; capacity to grow at acid, neutral, and alkaline pH; growth on LC medium; growth at 28 and 37 °C; melanin production capacity; Congo red absorption and symbiotic characteristics. These unbiased analyses of a total subset of strains isolated from specific soybean-cropping areas (an approach which could be called “
strainomics”) can be used to answer various biological questions. We illustrate this by a comparison of the molecular characteristics of five strains with interesting symbiotic properties. From this comparison we conclude, for instance, that differences in the efficiency of nitrogen fixation or competitiveness for nodulation of these strains are not apparently related to differences in Nod factor structure.