Postmeal Optogenetic Inhibition of Dorsal or Ventral Hippocampal Pyramidal Neurons Increases Future Intake

• Published in: eNeuro Vol. 6; no. 1; p. ENEURO.0457-18.2018
• Main Authors: Hannapel, Reilly, Ramesh, Janavi, Ross, Amy, LaLumiere, Ryan T, Roseberry, Aaron G, Parent, Marise B
• Format: Journal Article
• Language: English
• Published: United States Society for Neuroscience 01-01-2019
• Subjects: Animals; Confirmation; Dietary Sucrose; Eating - physiology; Feeding Behavior - physiology; Glutamic Acid - metabolism; Hippocampus - physiology; Memory - physiology; Neural Inhibition; Optogenetics; Postprandial Period - physiology; Pyramidal Cells - physiology; Rats, Sprague-Dawley; Saccharin; Tissue Culture Techniques; postprandial; memory; hippocampus; sucrose; feeding; saccharin
• ISSN: 2373-2822; 2373-2822
• DOI: 10.1523/eneuro.0457-18.2018

Memory of a recently eaten meal can serve as a powerful mechanism for controlling future eating behavior because it provides a record of intake that likely outlasts most physiological signals generated by the meal. In support, impairing the encoding of a meal in humans increases the amount ingested at the next eating episode. However, the brain regions that mediate the inhibitory effects of memory on future intake are unknown. In the present study, we tested the hypothesis that dorsal hippocampal (dHC) and ventral hippocampal (vHC) glutamatergic pyramidal neurons play a critical role in the inhibition of energy intake during the postprandial period by optogenetically inhibiting these neurons at specific times relative to a meal. Male Sprague Dawley rats were given viral vectors containing CaMKIIα-eArchT3.0-eYFP or CaMKIIα-GFP and fiber optic probes into dHC of one hemisphere and vHC of the other. Compared to intake on a day in which illumination was not given, inhibition of dHC or vHC glutamatergic neurons after the end of a chow, sucrose, or saccharin meal accelerated the onset of the next meal and increased the amount consumed during that next meal when the neurons were no longer inhibited. Inhibition given during a meal did not affect the amount consumed during that meal or the next one but did hasten meal initiation. These data show that dHC and vHC glutamatergic neuronal activity during the postprandial period is critical for limiting subsequent ingestion and suggest that these neurons inhibit future intake by consolidating the memory of the preceding meal.