Development of a murine model of chronic rhinosinusitis

• Published in: Otolaryngology-head and neck surgery Vol. 134; no. 5; pp. 724 - 730
• Main Authors: Lindsay, Robin, Slaughter, Tiffani, Britton-Webb, Joy, Mog, Steven R., Conran, Rich, Tadros, Monica, Earl, Natalie, Fox, David, Roberts, John, Bolger, William E.
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA Mosby, Inc 01-05-2006; SAGE Publications
• Subjects: Administration, Intranasal; Animals; Antigens, Fungal - administration & dosage; Antigens, Fungal - toxicity; Aspergillus fumigatus - immunology; Chronic Disease; Disease Models, Animal; Disease Progression; Eosinophilia - etiology; Eosinophilia - pathology; Female; Follow-Up Studies; Mice; Mice, Inbred BALB C; Rhinitis - etiology; Rhinitis - pathology; Sinusitis - etiology; Sinusitis - pathology
• ISSN: 0194-5998; 1097-6817
• DOI: 10.1016/j.otohns.2005.11.048

The aim of this study was to develop a mouse model of chronic eosinophilic rhinosinusitis.
Mice were sensitized to
Aspergillis fumigatus (Af) extract by intraperitoneal injection. The animals subsequently received nasal challenges with
Af extract 3 times per week for 12 weeks. Sinonasal complexes were studied histologically by the study otolaryngologists and pathologists to characterize the inflammatory response.
Animal care facility at an academic institution.
A chronic eosinophilic inflammatory response was evoked in all study animals. Statistical analysis was performed for inflammation, secretory cell hyperplasia, mast cells, and eosinophils. There were very significant differences (
P < 0.0005) between control and study mice in all categories.
Prolonged nasal challenge of
Af extract creates an inflammatory response in murine nasal mucosa that mimics human chronic eosinophilic rhinosinusitis.
A murine model for chronic rhinosinusitis is reported that may facilitate future investigations into disease pathophysiology.
EBM rating: B-2