Molecular identification and dynamics of microbial communities in reactor treating organic household waste

• Published in: Applied microbiology and biotechnology Vol. 84; no. 4; pp. 777 - 789
• Main Authors: Cardinali-Rezende, Juliana, Debarry, Renan B, Colturato, Luis F. D. B, Carneiro, Eduardo V, Chartone-Souza, Edmar, Nascimento, Andrea M. A
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-09-2009; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Actinobacteria; Alternative energy sources; Anaerobic digestion; Annealing; Archaea; Archaea - classification; Archaea - genetics; Archaea - isolation & purification; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Biodiversity; Biogas; Biological and medical sciences; Biological treatment of sewage sludges and wastes; Bioreactors - microbiology; Biotechnology; Cloning; Cluster Analysis; Cyanobacteria; Diversity indices; DNA, Archaeal - chemistry; DNA, Archaeal - genetics; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Environment and pollution; Environmental Biotechnology; Euryarchaeota; Family Characteristics; Firmicutes; Fundamental and applied biological sciences. Psychology; Gene amplification; Genes; Genes, rRNA; Household wastes; Households; Industrial applications and implications. Economical aspects; Leachates; Life Sciences; Medical Waste Disposal - methods; Methanobacterium; Methanoculleus; Methanomicrobiales; Microbial activity; Microbial Genetics and Genomics; Microbiology; Microorganisms; Molecular Sequence Data; Organic waste treatment; Organic wastes; Oxidoreductases - genetics; Phylogenetics; Phylogeny; Polymerase chain reaction; Proteobacteria; Reactors; Ribonucleic acid; RNA; RNA, Archaeal - genetics; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Startups; Studies; Time Factors; Waste disposal; Brazil; Clone library; Organic household waste; gene; Leachate; 16S rRNA gene; Anaerobic reactor; Organic waste; Waste treatment; Molecular dynamics; Identification; mcrA gene; Gene; Anaerobe; Ribosomal RNA; 16S-RNA; Reactor; Microbial community
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-009-2071-z

The prokaryotic diversity associated with organic household waste (OHW), leachate (start-up inoculum), and mesophilic anaerobic digestion processes in the degradation of OHW for 44 and 90 days was investigated using a culture-independent approach. Bacterial and archaeal 16S rRNA and mcrA gene clone libraries were constructed from community DNA preparations. Bacterial clones were affiliated with 13 phyla, of which Firmicutes, Proteobacteria, and Bacteroidetes were represented in all libraries, whereas Actinobacteria, Thermotogae, Lentisphaerae, Acidobacteria, Chloroflexi, Cyanobacteria, Synergistetes, Spirochaetes, Deferribacteres, and Deinococcus-Thermus were exclusively identified in a single library. Within the Archaea domain, the Euryarchaeota phylum was the only one represented. Corresponding sequences were associated with the following orders of hydrogenotrophic methanogens: Methanomicrobiales (Methanoculleus genus) and Methanobacteriales (Methanosphaera and Methanobacterium genera). One archaeal clone was not affiliated with any order and may represent a novel taxon. Diversity indices showed greater diversity of Bacteria when compared to methanogenic Archaea.