Snapshot Blotting: Transfer of Nucleic Acids and Nucleoprotein Complexes from Electrophoresis Gels to Grids for Electron Microscopy

We present a technique, "snapshot blotting," for the electrophoretic transfer of nucleic acids and nucleoprotein complexes in gel electrophoresis bands onto highly stable carbon film-coated grids for imaging by electron microscopy. The method permits structural analysis of macromolecular s...

Full description

Saved in:

Bibliographic Details
Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 91; no. 15; pp. 6870 - 6874
Main Authors:	Jett, Stephen D., Bear, David G.
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences of the United States of America 19-07-1994 National Acad Sciences National Academy of Sciences
Subjects:	Biochemistry Deoxyribonuclease EcoRI - ultrastructure Dimers DNA DNA - chemistry DNA - ultrastructure Dyes Electron microscopy Electrophoresis Electrophoresis, Agar Gel - methods Gels Microscopy, Electron Nucleic acids Nucleic Acids - chemistry Nucleic Acids - ultrastructure Nucleoproteins Nucleoproteins - chemistry Nucleoproteins - ultrastructure Proteins RNA Transcription, Genetic
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Be the first to leave a comment!