Escherichia coli “TatExpress” strains super‐secrete human growth hormone into the bacterial periplasm by the Tat pathway

Numerous high‐value proteins are secreted into the Escherichia coli periplasm by the General Secretory (Sec) pathway, but Sec‐based production chassis cannot handle many potential target proteins. The Tat pathway offers a promising alternative because it transports fully folded proteins; however, yi...

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Bibliographic Details
Published in:	Biotechnology and bioengineering Vol. 114; no. 12; pp. 2828 - 2836
Main Authors:	Browning, Douglas F., Richards, Kirsty L., Peswani, Amber R., Roobol, Jo, Busby, Stephen J.W., Robinson, Colin
Format:	Journal Article
Language:	English
Published:	United States Wiley Subscription Services, Inc 01-12-2017 John Wiley and Sons Inc
Subjects:	Bacteria biopharmaceutical production Chassis E coli Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Gene Products, tat - genetics Genetic Enhancement - methods Growth Hormone - biosynthesis Growth Hormone - genetics Growth Hormone - isolation & purification Growth hormones human growth hormone Humans Metabolic Networks and Pathways - genetics Periplasm Periplasm - metabolism Promoter Regions, Genetic - genetics protein secretion Proteins Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Secretion Secretory Pathway - genetics Strains (organisms) Tat human growth hormone Tat protein secretion biopharmaceutical production
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