2D FT-ICR MS of Calmodulin: A Top-Down and Bottom-Up Approach

• Published in: Journal of the American Society for Mass Spectrometry Vol. 27; no. 9; pp. 1531 - 1538
• Main Authors: Floris, Federico, van Agthoven, Maria, Chiron, Lionel, Soulby, Andrew J., Wootton, Christopher A., Lam, Yuko P. Y., Barrow, Mark P., Delsuc, Marc-André, O’Connor, Peter B.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-09-2016; Springer Nature B.V
• Subjects: Analytical Chemistry; Beta decay; Bioinformatics; Biotechnology; Calmodulin; Chemical Sciences; Chemistry; Chemistry and Materials Science; Cyclotron resonance; Electron capture; Fourier transforms; Fragmentation; Ions; Mass spectrometry; Organic Chemistry; Proteomics; Research Article; Scientific imaging; Spectroscopy; Tandem mass spectrometry; 2-Dimensional mass spectrometry; FTICR mass spectrometry
• ISSN: 1044-0305; 1879-1123; 1879-1123
• DOI: 10.1007/s13361-016-1431-z

Two-dimensional Fourier transform ion cyclotron resonance mass spectrometry (2D FT-ICR MS) allows data-independent fragmentation of all ions in a sample and correlation of fragment ions to their precursors through the modulation of precursor ion cyclotron radii prior to fragmentation. Previous results show that implementation of 2D FT-ICR MS with infrared multi-photon dissociation (IRMPD) and electron capture dissociation (ECD) has turned this method into a useful analytical tool. In this work, IRMPD tandem mass spectrometry of calmodulin (CaM) has been performed both in one-dimensional and two-dimensional FT-ICR MS using a top-down and bottom-up approach. 2D IRMPD FT-ICR MS is used to achieve extensive inter-residue bond cleavage and assignment for CaM, using its unique features for fragment identification in a less time- and sample-consuming experiment than doing the same thing using sequential MS/MS experiments.
Graphical Abstract
ᅟ