Neutrophil Elastase, an Innate Immunity Effector Molecule, Represses Flagellin Transcription in Pseudomonas aeruginosa

• Published in: Infection and Immunity Vol. 74; no. 12; pp. 6682 - 6689
• Main Authors: Sonawane, Avinash, Jyot, Jeevan, During, Russell, Ramphal, Reuben
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-12-2006
• Subjects: Bacteriology; Biological and medical sciences; Cell Extracts - immunology; Cell Extracts - pharmacology; Cystic Fibrosis - enzymology; Cystic Fibrosis - immunology; Flagellin - antagonists & inhibitors; Flagellin - biosynthesis; Flagellin - genetics; Fundamental and applied biological sciences. Psychology; Hot Temperature; Humans; Immunity, Innate; Leukocyte Elastase - metabolism; Microbiology; Miscellaneous; Molecular Pathogenesis; Mucus - enzymology; Mucus - immunology; Mucus - microbiology; Neutrophils - enzymology; Neutrophils - immunology; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - ultrastructure; Serine Endopeptidases - metabolism; Transcription, Genetic - drug effects; Pseudomonadales; Leukocyte elastase; Serine endopeptidases; Microbiology; Transcription; Enzyme; Flagellin; Natural immunity; Peptidases; Bacteria; Hydrolases; Pseudomonadaceae; Pseudomonas aeruginosa
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.00922-06

Recognition of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors triggers an innate immune response to colonizing or invading bacteria. Conversely, many bacteria have evolved mechanisms to dampen this response by downregulating the synthesis of such PAMPs. We have previously demonstrated that Pseudomonas aeruginosa growing in mucopurulent human respiratory mucus from cystic fibrosis patients represses the expression of its flagellin, a potent stimulant of the innate immune response. Here we demonstrate that this phenomenon occurs in response to the presence of neutrophil elastase in such mucus. Nonpurulent mucus from animals had no such repressive effect. Furthermore, lysed neutrophils from human blood reproduced the flagellin-repressive effect ex mucus and, significantly, had no effect on the viability of this organism. Neutrophil elastase, a component of the innate host defense system, has been described to be bactericidal for gram-negative bacteria and to degrade bacterial virulence factors. Thus, the resistance of P. aeruginosa to the bactericidal effect of neutrophil elastase, as well as this organism's ability to sense this enzyme's presence and downregulate the synthesis of a PAMP, may be the key factors in allowing P. aeruginosa to colonize the lungs. These findings demonstrate the dynamic nature of this bacterium's response to host defenses that ensures its success as a colonizer and also highlights the dual nature of defense molecules that confer advantages and disadvantages to both hosts and pathogens.