Studies on the Metabolism of Gomisin A (TJN-101). II. : Structure Determination of Biliary and Urinary Metabolites in Rat

• Published in: Chemical & pharmaceutical bulletin Vol. 38; no. 1; pp. 136 - 141
• Main Authors: IKEYA, Yukinobu, MITSUHASHI, Hiroshi, SASAKI, Hiromi, MATSUZAKI, Yutaka, MATSUZAKI, Tamae, HOSOYA, Eikichi
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1990; Maruzen
• Subjects: Animals; bile; Bile - metabolism; Biological and medical sciences; Cyclooctanes; Digestive system; Dioxoles; gomisin A; lignan; Lignans; Male; Medical sciences; metabolite; metabolitsm; Pharmacology. Drug treatments; Polycyclic Compounds - metabolism; Polycyclic Compounds - urine; rat; Rats; Rats, Inbred Strains; Schizandra chinensis; TJN-101; urine; Urine; Rat; Molecular structure; Metabolite; Rodentia; Oral administration; Metabolism; Vertebrata; Mammalia; Dicotyledones; Animal; Angiospermae; Plant origin; Spermatophyta; Pharmacokinetics; Schisandraceae; Bile
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.38.136

After oral administration of gomisin A (1) to rats, the bile and urine were collected and treated with β-glucuronidase and arylsulfatase. Seven metabolites, met B (2), met A-III (3), met E (4), met D (5), met F (6), met G (7), and met H (8) were isolated from the bile treated with the enzymes. Eight metabolites 2-8, and met A-II (9) were isolated from the urine treated with the enzymes. A major metabolite 2, and two minor metabolites 3 and 9 were identified as met B, met A-III, and met A-II, respectively, which are oxidative products of 1 formed by rat liver S9 mix. The structures of five new metabolites 4-7, and 8 were determined on the basis of chamical and spectral studies.