Clonal variability among oral Candida albicans assessed by allozyme electrophoresis analysis

• Published in: Oral microbiology and immunology Vol. 15; no. 6; pp. 350 - 354
• Main Authors: Mata, A. L., Rosa, R. T., Rosa, E. A. R., Gonçalves, R. B., Höfling, J. F.
• Format: Journal Article
• Language: English
• Published: Copenhagen Munksgaard International Publishers 01-12-2000; Blackwell
• Subjects: Biological and medical sciences; Brazil; Candida albicans; Candida albicans - enzymology; Candida albicans - genetics; Candida albicans - isolation & purification; Child; clonal analysis; Dentistry; Electrophoresis, Starch Gel; Fundamental and applied biological sciences. Psychology; Fungal Proteins - analysis; Gene Frequency; Genetic Variation; genotyping; Heterozygote; Humans; Microbiology; Mycology; oral colonization; Pathogenicity, host-agent relations, miscellaneous strains, epidemiology; Saliva - microbiology; South America; Brazil; America; Human; Candida albicans; Yeast; Healthy subject; Gel electrophoresis; Genotype; Microflora; Allozyme; Fungi; Oral cavity; Molecular epidemiology; Fungi Imperfecti; Child; Saliva; Thallophyta
• ISSN: 0902-0055; 1399-302X
• DOI: 10.1034/j.1399-302x.2000.150602.x

A total of 49 Candida albicans strains were isolated from the saliva of 11 healthy children in Piracicaba, Brazil and were analyzed according to their alloenzymatic patterns. Among eight loci assayed, seven were polymorphic and allowed to determine allelic and genotype frequencies, in order to establish the genetic variables for this fungal population. Some children showed just one genetic type, whereas other harbored two or more clones of such yeast, in a multiclonal manner of colonization by C. albicans.