Proliferation of osteoblast precursor cells on the surface of TiO2 nanowires anodically grown on a β-type biomedical titanium alloy

• Published in: Scientific reports Vol. 12; no. 1; p. 7895
• Main Authors: Fanton, Leonardo, Loria, Frida, Amores, Mario, Pazos, M. Ruth, Adán, Cristina, García-Muñoz, Rafael A., Marugán, Javier
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12-05-2022; Nature Publishing Group; Nature Portfolio
• Subjects: 639/166/898; 639/166/988; Biocompatibility; Bone growth; Cell growth; Cell proliferation; Cell survival; Cell viability; Colorimetry; Electrochemistry; Fluorescence microscopy; Humanities and Social Sciences; Microscopy; multidisciplinary; Nanotechnology; Nanotubes; Nanowires; Osteoblasts; Regeneration; Scanning electron microscopy; Science; Science (multidisciplinary); Titanium; Titanium alloys; Titanium dioxide
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-11981-4

Studies have shown that anodically grown TiO
2
nanotubes (TNTs) exhibit excellent biocompatibility. However, TiO
2
nanowires (TNWs) have received less attention. The objective of this study was to investigate the proliferation of osteoblast precursor cells on the surfaces of TNWs grown by electrochemical anodization of a Ti-35Nb-7Zr-5Ta (TNZT) alloy. TNT and flat TNZT surfaces were used as control samples. MC3T3-E1 cells were cultured on the surfaces of the samples for up to 5 days, and cell viability and proliferation were investigated using fluorescence microscopy, colorimetric assay, and scanning electron microscopy. The results showed lower cell proliferation rates on the TNW surface compared to control samples without significant differences in cell survival among experimental conditions. Contact angles measurements showed a good level of hydrophilicity for the TNWs, however, their relatively thin diameter and their high density may have affected cell proliferation. Although more research is necessary to understand all the parameters affecting biocompatibility, these TiO
2
nanostructures may represent promising tools for the treatment of bone defects and regeneration of bone tissue, among other applications.