Blue Dextran-Sepharose: An Affinity Column for the Dinucleotide Fold in Proteins

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 72; no. 2; pp. 669 - 672
• Main Authors: Thompson, Sioe Thung, Cass, Kathleen Heelan, Stellwagen, Earle
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 01-02-1975; National Acad Sciences
• Subjects: Binding Sites; Biochemistry; Chromatography, Affinity; Chromophores; Dehydrogenases; Dextrans; Elution; Enzymes; L-Lactate Dehydrogenase - isolation & purification; Lactates; Ligands; Models, Structural; NAD - metabolism; Nucleotides; Phosphofructokinase-1 - isolation & purification; Polysaccharides; Protein Binding; Protein Conformation; Proteins - isolation & purification; Ribonucleases - isolation & purification; Sepharose; Solvents
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.72.2.669

A procedure is described to utilize blue dextran-Sepharose as an affinity chromatographic column specific for the super-secondary structure called the dinucleotide fold, which forms the binding sites for substrates and effectors on a wide range of proteins. The procedure can be used to identify proteins, either purified or in crude cellular extracts, that possess the dinucleotide fold and to significantly improve the purification procedures for those proteins that possess the fold.