Polyphenol content and antioxidant activity of phytoestrogen containing food and dietary supplements: DPPH free radical scavenging activity by HPLC

• Published in: Acta Pharmaceutica Vol. 72; no. 3; pp. 375 - 388
• Main Authors: Klarić, Daniela Amidžić, Mornar, Ana, Kovačić, Jelena, Jeličić, Mario-Livio, Brusač, Edvin, Brletić, Iva, Klarić, Ilija
• Format: Journal Article Paper
• Language: English
• Published: Poland Sciendo 01-09-2022; De Gruyter Poland; Hrvatsko farmaceutsko društvo
• Subjects: antioxidant activity; Antioxidants; Antioxidants - chemistry; Chromatography, High Pressure Liquid; Dietary supplements; Dietary Supplements - analysis; Flax; Food; Food sources; Free Radicals; functional food; Functional foods & nutraceuticals; High-performance liquid chromatography; HPLC; Liquid chromatography; Metabolites; Oxidants; Oxidizing agents; Phenolic compounds; Phenols; Phytoestrogens; Plant Extracts - chemistry; polyphenol content; Polyphenols; Red clover; Scavenging; Secondary metabolites; Statistical analysis; Statistical methods; Trifolium pratense; dietary supplements; polyphenol content; functional food; phytoestrogens; antioxidant activity; HPLC
• ISSN: 1846-9558; 1330-0075; 1846-9558
• DOI: 10.2478/acph-2022-0031

Soy, red clover, chaste tree, hop and flax have all been found to contain a wide range of phytoestrogenic compounds, and a large number of dietary supplements contain their extracts as principal ingredients. This study is aimed to evaluate the total polyphenolic content and antioxidant activity of phytoestrogen-containing food and formulated dietary supplements. The HPLC-DPPH method was applied for DPPH free radical scavenging activity testing of various phytoestrogen-containing samples. Polyphenol content and antioxidant activity in dietary supplements were higher than in functional food samples; multiple-botanical-source preparations showed higher polyphenol content and antioxidant activity than the mono-botanical counterparts. Furthermore, the correlation between polyphenol content and anti-oxidant activity was strongly statistically significant, so it might be concluded that antioxidant activity is proportional to the content of these secondary metabolites. The most striking batch-to-batch deviations were represented by one chaste berry-based product (RSD 41.3 %) and one red clover derived product (RSD 57.9 %). The results of this study contribute to a better understanding of the phenolic profile and antioxidant properties of phytoestrogen containing food and dietary supplements.