The photosystem I supercomplex from a primordial green alga Ostreococcus tauri harbors three light-harvesting complex trimers

As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, is a model prasinophyte species for studying the functional evolution of the light-harvesting systems in photosynthesis. Here, we report the structure and function of the photosystem I (PSI) supercomplex in low light c...

Full description

Saved in:

Bibliographic Details
Published in:	eLife Vol. 12
Main Authors:	Ishii, Asako, Shan, Jianyu, Sheng, Xin, Kim, Eunchul, Watanabe, Akimasa, Yokono, Makio, Noda, Chiyo, Song, Chihong, Murata, Kazuyoshi, Liu, Zhenfeng, Minagawa, Jun
Format:	Journal Article
Language:	English
Published:	England eLife Sciences Publications, Ltd 23-03-2023 eLife Sciences Publications Ltd
Subjects:	Chlorophyll Chlorophyta - metabolism Computational and Systems Biology light-harvesting complex Light-Harvesting Protein Complexes - chemistry Light-Harvesting Protein Complexes - metabolism Ostreococcus tauri phosphorylation Photosynthesis photosystem I Photosystem I Protein Complex - chemistry Plant Biology prasinophyte state transitions computational biology light-harvesting complex systems biology prasinophyte Ostreococcus tauri photosystem I plant biology phosphorylation state transitions
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, is a model prasinophyte species for studying the functional evolution of the light-harvesting systems in photosynthesis. Here, we report the structure and function of the photosystem I (PSI) supercomplex in low light conditions, where it expands its photon-absorbing capacity by assembling with the light-harvesting complexes I (LHCI) and a prasinophyte-specific light-harvesting complex (Lhcp). The architecture of the supercomplex exhibits hybrid features of the plant-type and the green algal-type PSI supercomplexes, consisting of a PSI core, an Lhca1-Lhca4-Lhca2-Lhca3 belt attached on one side and an Lhca5-Lhca6 heterodimer associated on the other side between PsaG and PsaH. Interestingly, nine Lhcp subunits, including one Lhcp1 monomer with a phosphorylated amino-terminal threonine and eight Lhcp2 monomers, oligomerize into three trimers and associate with PSI on the third side between Lhca6 and PsaK. The Lhcp1 phosphorylation and the light-harvesting capacity of PSI were subjected to reversible photoacclimation, suggesting that the formation of PSI-LHCI-Lhcp supercomplex is likely due to a phosphorylation-dependent mechanism induced by changes in light intensity. Notably, this supercomplex did not exhibit far-red peaks in the 77 K fluorescence spectra, which is possibly due to the weak coupling of the chlorophyll 603- 609 pair in Lhca1-4.
AbstractList	As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, Ostreococcus tauri is a model prasinophyte species for studying the functional evolution of the light-harvesting systems in photosynthesis. Here, we report the structure and function of the O. tauri photosystem I (PSI) supercomplex in low light conditions, where it expands its photon-absorbing capacity by assembling with the light-harvesting complexes I (LHCI) and a prasinophyte-specific light-harvesting complex (Lhcp). The architecture of the supercomplex exhibits hybrid features of the plant-type and the green algal-type PSI supercomplexes, consisting of a PSI core, an Lhca1-Lhca4-Lhca2-Lhca3 belt attached on one side and an Lhca5-Lhca6 heterodimer associated on the other side between PsaG and PsaH. Interestingly, nine Lhcp subunits, including one Lhcp1 monomer with a phosphorylated amino-terminal threonine and eight Lhcp2 monomers, oligomerize into three trimers and associate with PSI on the third side between Lhca6 and PsaK. The Lhcp1 phosphorylation and the light-harvesting capacity of PSI were subjected to reversible photoacclimation, suggesting that the formation of Ot PSI-LHCI-Lhcp supercomplex is likely due to a phosphorylation-dependent mechanism induced by changes in light intensity. Notably, this supercomplex did not exhibit far-red peaks in the 77 K fluorescence spectra, which is possibly due to the weak coupling of the chlorophyll a 603- a 609 pair in Ot Lhca1-4. As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, Ostreococcus tauri is a model prasinophyte species for studying the functional evolution of the light-harvesting systems in photosynthesis. Here, we report the structure and function of the O. tauri photosystem I (PSI) supercomplex in low light conditions, where it expands its photon-absorbing capacity by assembling with the light-harvesting complexes I (LHCI) and a prasinophyte-specific light-harvesting complex (Lhcp). The architecture of the supercomplex exhibits hybrid features of the plant-type and the green algal-type PSI supercomplexes, consisting of a PSI core, an Lhca1-Lhca4-Lhca2-Lhca3 belt attached on one side and an Lhca5-Lhca6 heterodimer associated on the other side between PsaG and PsaH. Interestingly, nine Lhcp subunits, including one Lhcp1 monomer with a phosphorylated amino-terminal threonine and eight Lhcp2 monomers, oligomerize into three trimers and associate with PSI on the third side between Lhca6 and PsaK. The Lhcp1 phosphorylation and the light-harvesting capacity of PSI were subjected to reversible photoacclimation, suggesting that the formation of OtPSI-LHCI-Lhcp supercomplex is likely due to a phosphorylation-dependent mechanism induced by changes in light intensity. Notably, this supercomplex did not exhibit far-red peaks in the 77 K fluorescence spectra, which is possibly due to the weak coupling of the chlorophyll a603-a609 pair in OtLhca1-4. As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, is a model prasinophyte species for studying the functional evolution of the light-harvesting systems in photosynthesis. Here, we report the structure and function of the photosystem I (PSI) supercomplex in low light conditions, where it expands its photon-absorbing capacity by assembling with the light-harvesting complexes I (LHCI) and a prasinophyte-specific light-harvesting complex (Lhcp). The architecture of the supercomplex exhibits hybrid features of the plant-type and the green algal-type PSI supercomplexes, consisting of a PSI core, an Lhca1-Lhca4-Lhca2-Lhca3 belt attached on one side and an Lhca5-Lhca6 heterodimer associated on the other side between PsaG and PsaH. Interestingly, nine Lhcp subunits, including one Lhcp1 monomer with a phosphorylated amino-terminal threonine and eight Lhcp2 monomers, oligomerize into three trimers and associate with PSI on the third side between Lhca6 and PsaK. The Lhcp1 phosphorylation and the light-harvesting capacity of PSI were subjected to reversible photoacclimation, suggesting that the formation of PSI-LHCI-Lhcp supercomplex is likely due to a phosphorylation-dependent mechanism induced by changes in light intensity. Notably, this supercomplex did not exhibit far-red peaks in the 77 K fluorescence spectra, which is possibly due to the weak coupling of the chlorophyll 603- 609 pair in Lhca1-4.
Author	Sheng, Xin Murata, Kazuyoshi Liu, Zhenfeng Watanabe, Akimasa Song, Chihong Yokono, Makio Noda, Chiyo Minagawa, Jun Shan, Jianyu Ishii, Asako Kim, Eunchul
Author_xml	– sequence: 1 givenname: Asako surname: Ishii fullname: Ishii, Asako organization: Division of Environmental Photobiology, National Institute for Basic Biology, Okazaki, Japan – sequence: 2 givenname: Jianyu surname: Shan fullname: Shan, Jianyu organization: College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China – sequence: 3 givenname: Xin surname: Sheng fullname: Sheng, Xin organization: National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China – sequence: 4 givenname: Eunchul surname: Kim fullname: Kim, Eunchul organization: Department of Basic Biology, School of Life Science, the Graduate University for Advanced Studies, Okazaki, Japan – sequence: 5 givenname: Akimasa orcidid: 0000-0001-6068-1328 surname: Watanabe fullname: Watanabe, Akimasa organization: Department of Basic Biology, School of Life Science, the Graduate University for Advanced Studies, Okazaki, Japan – sequence: 6 givenname: Makio surname: Yokono fullname: Yokono, Makio organization: Department of Basic Biology, School of Life Science, the Graduate University for Advanced Studies, Okazaki, Japan – sequence: 7 givenname: Chiyo surname: Noda fullname: Noda, Chiyo organization: Division of Environmental Photobiology, National Institute for Basic Biology, Okazaki, Japan – sequence: 8 givenname: Chihong surname: Song fullname: Song, Chihong organization: Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Japan – sequence: 9 givenname: Kazuyoshi orcidid: 0000-0001-9446-3652 surname: Murata fullname: Murata, Kazuyoshi organization: Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Japan – sequence: 10 givenname: Zhenfeng orcidid: 0000-0001-5502-9474 surname: Liu fullname: Liu, Zhenfeng organization: College of Life Sciences, University of Chinese Academy of Sciences, Beijing, China – sequence: 11 givenname: Jun orcidid: 0000-0002-3028-3203 surname: Minagawa fullname: Minagawa, Jun organization: Department of Basic Biology, School of Life Science, the Graduate University for Advanced Studies, Okazaki, Japan
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/36951548$$D View this record in MEDLINE/PubMed
BookMark	eNpVkk1r3DAQhkVJadI0p96LjoWyqT4sWz6VEpp2YSGXFHoTsjyyFWTLleTQHPrfo80mIdFBH6OXZ0aa9z06msMMCH2k5LwRovoK3lk4l1Ul5Rt0woggGyKrP0cv9sfoLKUbUkZTVLR9h4553QoqKnmC_l-PgJcx5JDuUoYJb3FaF4gmTIuHf9jGMGGNl-imEHunPR4iwIy1HzS-SjlCMMGYNeGs1-jwqGMXYjmNRYa9G8a8KbFbSNnNA37C5sKDmD6gt1b7BGeP6yn6ffnj-uLXZnf1c3vxfbcxgla5zIR3pfq-bkQtGyNrabnsQDPGtGk0JZXgnW21ICAkbwE4F0zWzFjRA6X8FG0P3D7oG7V_jI53KminHgIhDkrH7IwHxVvLKG9q3fddJazVgoPsoSaENoQzUljfDqxl7SboDcw5av8K-vpmdqMawq2ihLRNxVghfH4kxPB3LT-jJpcMeK9nCGtSrGlJaZ6k-2RfDlITQ0oR7HMeStTeAAp2xQDqwQBF_ellac_ap3bze1IbsS0
CitedBy_id	crossref_primary_10_1016_j_bbabio_2023_148995 crossref_primary_10_1038_s41467_024_46791_x crossref_primary_10_1073_pnas_2315476121 crossref_primary_10_1016_j_molp_2023_12_016 crossref_primary_10_3390_ijms25052765
Cites_doi	10.1146/annurev-arplant-071720-015522 10.1038/s41477-018-0271-1 10.1007/s11120-016-0219-0 10.1074/jbc.M309203200 10.1038/nmeth.4169 10.1038/s41477-021-00960-8 10.1016/j.bbabio.2015.07.006 10.1111/j.1399-3054.1991.tb02490.x 10.1038/s41477-019-0438-4 10.1016/j.bbabio.2010.04.017 10.1074/jbc.M402348200 10.1002/anie.201512001 10.1107/S2059798318006551 10.1104/pp.104.2.797 10.7554/eLife.18722 10.1093/jxb/ert126 10.1007/s11120-013-9838-x 10.1016/j.jsb.2015.08.008 10.1073/pnas.96.18.10056 10.1073/pnas.0611046104 10.1104/pp.106.092536 10.1111/j.1751-1097.2009.00619.x 10.1038/s41467-021-21362-6 10.1038/nplants.2017.14 10.1093/molbev/msi220 10.1105/tpc.108.059352 10.1002/1873-3468.13394 10.1016/S0021-9258(19)47412-7 10.1021/bi036265i 10.1017/CBO9780511732263 10.1038/nature02373 10.1016/s0005-2728(00)00101-8 10.1016/j.bbabio.2009.10.005 10.3732/ajb.91.10.1535 10.1038/35082000 10.1093/pcp/pcz026 10.1021/jp044082f 10.1038/370255a0 10.1021/bi034975i 10.1038/nmeth.4193 10.1007/BF00034776 10.1038/s41477-019-0543-4 10.1016/j.jsb.2012.09.006 10.1016/B978-0-12-370873-1.00022-8 10.1038/s41421-021-00242-9 10.1111/j.1529-8817.2004.03136.x 10.1016/0003-9861(82)90535-5 10.1038/s41477-018-0270-2 10.1038/ncomms7675 10.1038/nprot.2006.468 10.1038/s41477-022-01099-w 10.1007/s11120-019-00669-y 10.1016/j.bbabio.2010.11.005 10.1039/c1cp21079c 10.1073/pnas.0604795103 10.1074/jbc.RA118.006536 10.1126/science.aab0214 10.1038/s41477-019-0380-5 10.1016/j.bbabio.2020.148253 10.1146/annurev-arplant-050213-040226 10.1126/science.aay2058 10.1126/science.aat1156 10.1073/pnas.1222606110 10.1073/pnas.1722482115 10.1021/bi962524a
ContentType	Journal Article
Copyright	2023, Ishii, Shan et al. 2023, Ishii, Shan et al 2023 Ishii, Shan et al
Copyright_xml	– notice: 2023, Ishii, Shan et al. – notice: 2023, Ishii, Shan et al 2023 Ishii, Shan et al
DBID	CGR CUY CVF ECM EIF NPM AAYXX CITATION 7X8 5PM DOA
DOI	10.7554/elife.84488
DatabaseName	Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef MEDLINE - Academic PubMed Central (Full Participant titles) Directory of Open Access Journals
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef MEDLINE - Academic
DatabaseTitleList	CrossRef MEDLINE
Database_xml	– sequence: 1 dbid: DOA name: Directory of Open Access Journals url: http://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: ECM name: MEDLINE url: https://search.ebscohost.com/login.aspx?direct=true&db=cmedm&site=ehost-live sourceTypes: Index Database
DeliveryMethod	fulltext_linktorsrc
EISSN	2050-084X
ExternalDocumentID	oai_doaj_org_article_39f21376addb45ffa53e8de600170320 10_7554_eLife_84488 36951548
Genre	Research Support, Non-U.S. Gov't Journal Article
GrantInformation_xml	– fundername: National Natural Science Foundation of China grantid: 31925024 – fundername: Chinese Academy of Sciences grantid: YSBR-015 – fundername: Japan Society for the Promotion of Science grantid: 21H04778 – fundername: Chinese Academy of Sciences grantid: XDB37020101 – fundername: Japan Society for the Promotion of Science grantid: 21H05040 – fundername: National Key Research and Development Program of China grantid: 2017YFA0503702 – fundername: ; grantid: YSBR-015 – fundername: ; grantid: 21H05040 – fundername: ; grantid: 2017YFA0503702 – fundername: ; grantid: 21H04778 – fundername: ; grantid: 31925024 – fundername: ; grantid: XDB37020101
GroupedDBID	3V. 53G 5VS 7X7 88E 88I 8FE 8FH 8FI 8FJ AAFWJ AAKDD ABUWG ACGFO ACGOD ACPRK ADBBV ADRAZ AENEX AFKRA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC BAWUL BBNVY BCNDV BENPR BHPHI BPHCQ BVXVI CCPQU CGR CUY CVF DIK DWQXO ECM EIF EMOBN FRP FYUFA GNUQQ GROUPED_DOAJ GX1 HCIFZ HMCUK HYE IAO IEA IHR INH INR ISR ITC KQ8 LK8 M1P M2P M48 M7P M~E NPM NQS OK1 PGMZT PIMPY PQQKQ PROAC PSQYO RHF RHI RNS RPM UKHRP AAYXX CITATION 7X8 5PM AFPKN
ID	FETCH-LOGICAL-c514t-c503b074d675687c868f38bea222ac7a10453bf9a50e5839ee3352862cf5de113
IEDL.DBID	RPM
ISSN	2050-084X
IngestDate	Tue Oct 22 15:14:59 EDT 2024 Tue Sep 17 21:33:06 EDT 2024 Sat Oct 05 05:40:07 EDT 2024 Fri Nov 22 01:10:21 EST 2024 Wed Oct 16 00:39:54 EDT 2024
IsDoiOpenAccess	false
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Keywords	computational biology light-harvesting complex systems biology prasinophyte Ostreococcus tauri photosystem I plant biology phosphorylation state transitions
Language	English
License	2023, Ishii, Shan et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c514t-c503b074d675687c868f38bea222ac7a10453bf9a50e5839ee3352862cf5de113
Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. Shenzhen Jingtai Technology Co. Ltd, Shenzhen, China.
ORCID	0000-0002-3028-3203 0000-0001-6068-1328 0000-0001-5502-9474 0000-0001-9446-3652
OpenAccessLink	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10097422/
PMID	36951548
PQID	2790050810
PQPubID	23479
ParticipantIDs	doaj_primary_oai_doaj_org_article_39f21376addb45ffa53e8de600170320 pubmedcentral_primary_oai_pubmedcentral_nih_gov_10097422 proquest_miscellaneous_2790050810 crossref_primary_10_7554_eLife_84488 pubmed_primary_36951548
PublicationCentury	2000
PublicationDate	2023-03-23
PublicationDateYYYYMMDD	2023-03-23
PublicationDate_xml	– month: 03 year: 2023 text: 2023-03-23 day: 23
PublicationDecade	2020
PublicationPlace	England
PublicationPlace_xml	– name: England
PublicationTitle	eLife
PublicationTitleAlternate	Elife
PublicationYear	2023
Publisher	eLife Sciences Publications, Ltd eLife Sciences Publications Ltd
Publisher_xml	– name: eLife Sciences Publications, Ltd – name: eLife Sciences Publications Ltd
References	Goss (bib13) 2000; 1457 Latasa (bib24) 2004; 40 O’Connor (bib37) 1984 Pan (bib39) 2018; 360 Sheng (bib50) 2019; 5 Minagawa (bib32) 2011; 1807 Croce (bib10) 2020; 369 Bassi (bib3) 2021; 72 Klughammer (bib21) 2016; 128 Pan (bib40) 2021; 7 Swingley (bib57) 2010; 1797 Watanabe (bib59) 2019; 593 Schmitt (bib49) 1994; 40 Wentworth (bib60) 2004; 43 Derelle (bib11) 2006; 103 Shevchenko (bib51) 2006; 1 Caspy (bib7) 2020; 1861 Yokono (bib63) 2015; 6 Tokutsu (bib58) 2013; 110 Caffarri (bib6) 2004; 43 Courties (bib8) 1994; 370 Bednarczyk (bib4) 2016; 55 Liu (bib26) 2004; 428 Bassi (bib2) 1999; 96 Michel (bib29) 1991; 266 Koziol (bib22) 2007; 143 Morosinotto (bib33) 2003; 278 Rochaix (bib45) 2014; 65 Iwai (bib16) 2018; 4 Qin (bib44) 2015; 348 Mazor (bib27) 2017; 3 Pi (bib41) 2018; 115 Gorski (bib12) 2022; 8 Young (bib65) 1991; 83 Jiao (bib17) 1994; 104 Jordan (bib18) 2001; 411 Mimuro (bib30) 2010; 86 Melis (bib28) 1982; 217 Minagawa (bib31) 2009 Standfuss (bib53) 2004; 279 Palenik (bib38) 2007; 104 Mozzo (bib34) 2010; 1797 Yokono (bib64) 2019; 60 Pinnola (bib42) 2018; 4 Punjani (bib43) 2017; 14 Kimanius (bib20) 2016; 5 Scheres (bib48) 2012; 180 Suga (bib56) 2019; 5 Iwai (bib15) 2008; 20 Huang (bib14) 2021; 12 Rohou (bib46) 2015; 192 Six (bib52) 2005; 22 Roy (bib47) 2011 Yan (bib61) 2021; 7 Novoderezhkin (bib35) 2005; 109 Croce (bib9) 2013; 116 Kubota-Kawai (bib23) 2019; 294 Novoderezhkin (bib36) 2011; 13 Su (bib55) 2019; 5 Afonine (bib1) 2018; 74 Kawakami (bib19) 2019; 142 Sturgis (bib54) 1997; 36 Yokono (bib62) 2015; 1847 Lewis (bib25) 2004; 91 Zheng (bib66) 2017; 14 Busch (bib5) 2013; 64
References_xml	– volume: 72 start-page: 47 year: 2021 ident: bib3 article-title: Dissipation of light energy absorbed in excess: the molecular mechanisms publication-title: Annual Review of Plant Biology doi: 10.1146/annurev-arplant-071720-015522 contributor: fullname: Bassi – volume: 4 start-page: 904 year: 2018 ident: bib16 article-title: A unique supramolecular organization of photosystem I in the moss Physcomitrella patens publication-title: Nature Plants doi: 10.1038/s41477-018-0271-1 contributor: fullname: Iwai – volume: 128 start-page: 195 year: 2016 ident: bib21 article-title: Deconvolution of ferredoxin, plastocyanin, and P700 transmittance changes in intact leaves with a new type of kinetic led array spectrophotometer publication-title: Photosynthesis Research doi: 10.1007/s11120-016-0219-0 contributor: fullname: Klughammer – volume: 278 start-page: 49223 year: 2003 ident: bib33 article-title: The nature of a chlorophyll ligand in lhca proteins determines the far red fluorescence emission typical of photosystem I publication-title: The Journal of Biological Chemistry doi: 10.1074/jbc.M309203200 contributor: fullname: Morosinotto – volume: 14 start-page: 290 year: 2017 ident: bib43 article-title: CryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination publication-title: Nature Methods doi: 10.1038/nmeth.4169 contributor: fullname: Punjani – volume: 7 start-page: 1119 year: 2021 ident: bib40 article-title: Structural basis of lhcbm5-mediated state transitions in green algae publication-title: Nature Plants doi: 10.1038/s41477-021-00960-8 contributor: fullname: Pan – volume: 1847 start-page: 1274 year: 2015 ident: bib62 article-title: Regulation of excitation energy transfer in diatom PSII dimer: how does it change the destination of excitation energy? publication-title: Biochimica et Biophysica Acta doi: 10.1016/j.bbabio.2015.07.006 contributor: fullname: Yokono – volume: 83 start-page: 702 year: 1991 ident: bib65 article-title: The photoprotective role of carotenoids in higher plants publication-title: Physiologia Plantarum doi: 10.1111/j.1399-3054.1991.tb02490.x contributor: fullname: Young – volume: 5 start-page: 626 year: 2019 ident: bib56 article-title: Structure of the green algal photosystem I supercomplex with a decameric light-harvesting complex I publication-title: Nature Plants doi: 10.1038/s41477-019-0438-4 contributor: fullname: Suga – volume: 1797 start-page: 1458 year: 2010 ident: bib57 article-title: Characterization of photosystem I antenna proteins in the prasinophyte ostreococcus tauri publication-title: Biochimica et Biophysica Acta doi: 10.1016/j.bbabio.2010.04.017 contributor: fullname: Swingley – volume: 279 start-page: 36884 year: 2004 ident: bib53 article-title: The three isoforms of the light-harvesting complex II: spectroscopic features, trimer formation, and functional roles publication-title: The Journal of Biological Chemistry doi: 10.1074/jbc.M402348200 contributor: fullname: Standfuss – volume: 55 start-page: 6901 year: 2016 ident: bib4 article-title: Fine tuning of chlorophyll spectra by protein-induced ring deformation publication-title: Angewandte Chemie doi: 10.1002/anie.201512001 contributor: fullname: Bednarczyk – volume: 74 start-page: 531 year: 2018 ident: bib1 article-title: Real-space refinement in PHENIX for cryo-EM and crystallography publication-title: Acta Crystallographica. Section D, Structural Biology doi: 10.1107/S2059798318006551 contributor: fullname: Afonine – volume: 104 start-page: 797 year: 1994 ident: bib17 article-title: A cdna clone encoding A light-harvesting protein from mantoniella squamata publication-title: Plant Physiology doi: 10.1104/pp.104.2.797 contributor: fullname: Jiao – volume: 5 year: 2016 ident: bib20 article-title: Accelerated cryo-EM structure determination with parallelisation using gpus in RELION-2 publication-title: eLife doi: 10.7554/eLife.18722 contributor: fullname: Kimanius – volume: 64 start-page: 2689 year: 2013 ident: bib5 article-title: Composition and structure of photosystem I in the moss Physcomitrella patens publication-title: Journal of Experimental Botany doi: 10.1093/jxb/ert126 contributor: fullname: Busch – volume: 116 start-page: 153 year: 2013 ident: bib9 article-title: Light-harvesting in photosystem I publication-title: Photosynthesis Research doi: 10.1007/s11120-013-9838-x contributor: fullname: Croce – volume: 192 start-page: 216 year: 2015 ident: bib46 article-title: CTFFIND4: fast and accurate defocus estimation from electron micrographs publication-title: Journal of Structural Biology doi: 10.1016/j.jsb.2015.08.008 contributor: fullname: Rohou – volume: 96 start-page: 10056 year: 1999 ident: bib2 article-title: Mutational analysis of a higher plant antenna protein provides identification of chromophores bound into multiple sites publication-title: PNAS doi: 10.1073/pnas.96.18.10056 contributor: fullname: Bassi – volume: 104 start-page: 7705 year: 2007 ident: bib38 article-title: The tiny eukaryote Ostreococcus provides genomic insights into the paradox of plankton speciation publication-title: PNAS doi: 10.1073/pnas.0611046104 contributor: fullname: Palenik – volume: 143 start-page: 1802 year: 2007 ident: bib22 article-title: Tracing the evolution of the light-harvesting antennae in chlorophyll a/b-containing organisms publication-title: Plant Physiology doi: 10.1104/pp.106.092536 contributor: fullname: Koziol – volume: 86 start-page: 62 year: 2010 ident: bib30 article-title: Variations in photosystem I properties in the primordial cyanobacterium gloeobacter violaceus PCC 7421 publication-title: Photochemistry and Photobiology doi: 10.1111/j.1751-1097.2009.00619.x contributor: fullname: Mimuro – volume: 12 year: 2021 ident: bib14 article-title: Structure of photosystem I-LHCI-LHCII from the green alga Chlamydomonas reinhardtii in state 2 publication-title: Nature Communications doi: 10.1038/s41467-021-21362-6 contributor: fullname: Huang – volume: 3 year: 2017 ident: bib27 article-title: Structure of the plant photosystem I supercomplex at 2.6 å resolution publication-title: Nature Plants doi: 10.1038/nplants.2017.14 contributor: fullname: Mazor – volume: 22 start-page: 2217 year: 2005 ident: bib52 article-title: New insights into the nature and phylogeny of prasinophyte antenna proteins: ostreococcus tauri, a case study publication-title: Molecular Biology and Evolution doi: 10.1093/molbev/msi220 contributor: fullname: Six – volume: 20 start-page: 2177 year: 2008 ident: bib15 article-title: Molecular remodeling of photosystem II during state transitions in Chlamydomonas reinhardtii publication-title: The Plant Cell doi: 10.1105/tpc.108.059352 contributor: fullname: Iwai – volume: 593 start-page: 1072 year: 2019 ident: bib59 article-title: Amphipol-assisted purification method for the highly active and stable photosystem II supercomplex of Chlamydomonas reinhardtii publication-title: FEBS Letters doi: 10.1002/1873-3468.13394 contributor: fullname: Watanabe – volume: 266 start-page: 17584 year: 1991 ident: bib29 article-title: Tandem mass spectrometry identifies sites of three post-translational modifications of spinach light-harvesting chlorophyll protein II. proteolytic cleavage, acetylation, and phosphorylation publication-title: The Journal of Biological Chemistry doi: 10.1016/S0021-9258(19)47412-7 contributor: fullname: Michel – volume: 43 start-page: 9467 year: 2004 ident: bib6 article-title: A look within LHCII: differential analysis of the lhcb1-3 complexes building the major trimeric antenna complex of higher-plant photosynthesis publication-title: Biochemistry doi: 10.1021/bi036265i contributor: fullname: Caffarri – volume-title: Phytoplankton Pigments: Characterization, Chemotaxonomy and Applications in Oceanography year: 2011 ident: bib47 doi: 10.1017/CBO9780511732263 contributor: fullname: Roy – volume: 428 start-page: 287 year: 2004 ident: bib26 article-title: Crystal structure of spinach major light-harvesting complex at 2.72 A resolution publication-title: Nature doi: 10.1038/nature02373 contributor: fullname: Liu – volume: 1457 start-page: 190 year: 2000 ident: bib13 article-title: Organization of the pigment molecules in the chlorophyll a/b/c containing alga mantoniella squamata (prasinophyceae) studied by means of absorption, circular and linear dichroism spectroscopy publication-title: Biochimica et Biophysica Acta doi: 10.1016/s0005-2728(00)00101-8 contributor: fullname: Goss – volume: 1797 start-page: 212 year: 2010 ident: bib34 article-title: Functional analysis of photosystem I light-harvesting complexes (lhca) gene products of Chlamydomonas reinhardtii publication-title: Biochimica et Biophysica Acta doi: 10.1016/j.bbabio.2009.10.005 contributor: fullname: Mozzo – volume: 91 start-page: 1535 year: 2004 ident: bib25 article-title: Green algae and the origin of land plants publication-title: American Journal of Botany doi: 10.3732/ajb.91.10.1535 contributor: fullname: Lewis – volume: 411 start-page: 909 year: 2001 ident: bib18 article-title: Three-dimensional structure of cyanobacterial photosystem I at 2.5 A resolution publication-title: Nature doi: 10.1038/35082000 contributor: fullname: Jordan – volume: 60 start-page: 1098 year: 2019 ident: bib64 article-title: The PSI-PSII megacomplex in green plants publication-title: Plant and Cell Physiology doi: 10.1093/pcp/pcz026 contributor: fullname: Yokono – volume: 109 start-page: 10493 year: 2005 ident: bib35 article-title: Excitation dynamics in the LHCII complex of higher plants: modeling based on the 2.72 angstrom crystal structure publication-title: The Journal of Physical Chemistry. B doi: 10.1021/jp044082f contributor: fullname: Novoderezhkin – volume: 370 year: 1994 ident: bib8 article-title: Smallest eukaryotic organism publication-title: Nature doi: 10.1038/370255a0 contributor: fullname: Courties – volume: 43 start-page: 501 year: 2004 ident: bib60 article-title: The functional significance of the monomeric and trimeric states of the photosystem II light harvesting complexes publication-title: Biochemistry doi: 10.1021/bi034975i contributor: fullname: Wentworth – volume: 14 start-page: 331 year: 2017 ident: bib66 article-title: MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy publication-title: Nature Methods doi: 10.1038/nmeth.4193 contributor: fullname: Zheng – volume: 40 start-page: 269 year: 1994 ident: bib49 article-title: Polypeptide sequence of the chlorophyll a/b/c-binding protein of the prasinophycean alga mantoniella squamata publication-title: Photosynthesis Research doi: 10.1007/BF00034776 contributor: fullname: Schmitt – volume: 5 start-page: 1320 year: 2019 ident: bib50 article-title: Structural insight into light harvesting for photosystem II in green algae publication-title: Nature Plants doi: 10.1038/s41477-019-0543-4 contributor: fullname: Sheng – volume: 180 start-page: 519 year: 2012 ident: bib48 article-title: RELION: implementation of a bayesian approach to cryo-EM structure determination publication-title: Journal of Structural Biology doi: 10.1016/j.jsb.2012.09.006 contributor: fullname: Scheres – start-page: 503 volume-title: The Chlamydomonas Sourcebook year: 2009 ident: bib31 doi: 10.1016/B978-0-12-370873-1.00022-8 contributor: fullname: Minagawa – volume: 7 year: 2021 ident: bib61 article-title: Antenna arrangement and energy-transfer pathways of PSI-LHCI from the moss Physcomitrella patens publication-title: Cell Discovery doi: 10.1038/s41421-021-00242-9 contributor: fullname: Yan – volume: 40 start-page: 1149 year: 2004 ident: bib24 article-title: PIGMENT suites and taxonomic groups in prasinophyceae publication-title: Journal of Phycology doi: 10.1111/j.1529-8817.2004.03136.x contributor: fullname: Latasa – volume-title: Time-Correlated Single Photon Counting year: 1984 ident: bib37 contributor: fullname: O’Connor – volume: 217 start-page: 536 year: 1982 ident: bib28 article-title: Kinetic analysis of P-700 photoconversion: effect of secondary electron donation and plastocyanin inhibition publication-title: Archives of Biochemistry and Biophysics doi: 10.1016/0003-9861(82)90535-5 contributor: fullname: Melis – volume: 4 start-page: 910 year: 2018 ident: bib42 article-title: A LHCB9-dependent photosystem I megacomplex induced under low light in Physcomitrella patens publication-title: Nature Plants doi: 10.1038/s41477-018-0270-2 contributor: fullname: Pinnola – volume: 6 year: 2015 ident: bib63 article-title: A megacomplex composed of both photosystem reaction centres in higher plants publication-title: Nature Communications doi: 10.1038/ncomms7675 contributor: fullname: Yokono – volume: 1 start-page: 2856 year: 2006 ident: bib51 article-title: In-gel digestion for mass spectrometric characterization of proteins and proteomes publication-title: Nature Protocols doi: 10.1038/nprot.2006.468 contributor: fullname: Shevchenko – volume: 8 start-page: 307 year: 2022 ident: bib12 article-title: The structure of the physcomitrium patens photosystem I reveals a unique Lhca2 paralogue replacing Lhca4 publication-title: Nature Plants doi: 10.1038/s41477-022-01099-w contributor: fullname: Gorski – volume: 142 start-page: 195 year: 2019 ident: bib19 article-title: Four distinct trimeric forms of light-harvesting complex II isolated from the green alga Chlamydomonas reinhardtii publication-title: Photosynthesis Research doi: 10.1007/s11120-019-00669-y contributor: fullname: Kawakami – volume: 1807 start-page: 897 year: 2011 ident: bib32 article-title: State transitions--the molecular remodeling of photosynthetic supercomplexes that controls energy flow in the chloroplast publication-title: Biochimica et Biophysica Acta doi: 10.1016/j.bbabio.2010.11.005 contributor: fullname: Minagawa – volume: 13 start-page: 17093 year: 2011 ident: bib36 article-title: Intra- and inter-monomeric transfers in the light harvesting LHCII complex: the redfield-Förster picture publication-title: Physical Chemistry Chemical Physics doi: 10.1039/c1cp21079c contributor: fullname: Novoderezhkin – volume: 103 start-page: 11647 year: 2006 ident: bib11 article-title: Genome analysis of the smallest free-living eukaryote ostreococcus tauri unveils many unique features publication-title: PNAS doi: 10.1073/pnas.0604795103 contributor: fullname: Derelle – volume: 294 start-page: 4304 year: 2019 ident: bib23 article-title: Ten antenna proteins are associated with the core in the supramolecular organization of the photosystem I supercomplex in Chlamydomonas reinhardtii publication-title: The Journal of Biological Chemistry doi: 10.1074/jbc.RA118.006536 contributor: fullname: Kubota-Kawai – volume: 348 start-page: 989 year: 2015 ident: bib44 article-title: Photosynthesis. Structural basis for energy transfer pathways in the plant PSI-LHCI supercomplex publication-title: Science doi: 10.1126/science.aab0214 contributor: fullname: Qin – volume: 5 start-page: 273 year: 2019 ident: bib55 article-title: Antenna arrangement and energy transfer pathways of a green algal photosystem-I-LHCI supercomplex publication-title: Nature Plants doi: 10.1038/s41477-019-0380-5 contributor: fullname: Su – volume: 1861 year: 2020 ident: bib7 article-title: Structure and energy transfer pathways of the dunaliella salina photosystem I supercomplex publication-title: Biochimica et Biophysica Acta. Bioenergetics doi: 10.1016/j.bbabio.2020.148253 contributor: fullname: Caspy – volume: 65 start-page: 287 year: 2014 ident: bib45 article-title: Regulation and dynamics of the light-harvesting system publication-title: Annual Review of Plant Biology doi: 10.1146/annurev-arplant-050213-040226 contributor: fullname: Rochaix – volume: 369 year: 2020 ident: bib10 article-title: Light harvesting in oxygenic photosynthesis: structural biology meets spectroscopy publication-title: Science doi: 10.1126/science.aay2058 contributor: fullname: Croce – volume: 360 start-page: 1109 year: 2018 ident: bib39 article-title: Structure of the maize photosystem I supercomplex with light-harvesting complexes I and II publication-title: Science doi: 10.1126/science.aat1156 contributor: fullname: Pan – volume: 110 start-page: 10016 year: 2013 ident: bib58 article-title: Energy-dissipative supercomplex of photosystem ii associated with lhcsr3 in Chlamydomonas reinhardtii publication-title: PNAS doi: 10.1073/pnas.1222606110 contributor: fullname: Tokutsu – volume: 115 start-page: 4423 year: 2018 ident: bib41 article-title: Unique organization of photosystem I-light-harvesting supercomplex revealed by cryo-EM from a red alga publication-title: PNAS doi: 10.1073/pnas.1722482115 contributor: fullname: Pi – volume: 36 start-page: 2772 year: 1997 ident: bib54 article-title: Functions of conserved tryptophan residues of the core light-harvesting complex of rhodobacter sphaeroides publication-title: Biochemistry doi: 10.1021/bi962524a contributor: fullname: Sturgis
SSID	ssj0000748819
Score	2.4401402
Snippet	As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, is a model prasinophyte species for studying the functional evolution of the... As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, Ostreococcus tauri is a model prasinophyte species for studying the... As a ubiquitous picophytoplankton in the ocean and an early-branching green alga, Ostreococcus tauri is a model prasinophyte species for studying the...
SourceID	doaj pubmedcentral proquest crossref pubmed
SourceType	Open Website Open Access Repository Aggregation Database Index Database
SubjectTerms	Chlorophyll Chlorophyta - metabolism Computational and Systems Biology light-harvesting complex Light-Harvesting Protein Complexes - chemistry Light-Harvesting Protein Complexes - metabolism Ostreococcus tauri phosphorylation Photosynthesis photosystem I Photosystem I Protein Complex - chemistry Plant Biology prasinophyte state transitions
SummonAdditionalLinks	– databaseName: Directory of Open Access Journals dbid: DOA link: http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LS8QwEA66Jy-i-FpfRPBa3TZtmh59oiB6UMFbSdKJK0i79AFe_O_OpLuLK4IXL4WmoQ3z5TEfnfmGseNCWZ1ZivOTWRHEUkKgU20CmznQSo9AOMpGvnlM71_U5RXJ5MxLfVFMWC8P3BvuVGQuCnEV4Do0ceKcTgSoAqQXfhFRz9ZH8huZ8ntwihMzzPqEvBSPzFO4e3NwopCNqIUjyCv1_-Ze_oyS_HbsXK-x1am_yM_6ca6zJSg32CeCyyfjqq16IWZ-y5tuArWPD4cPTjkjXPNJjUDQBHjnrxRewylvgz9QekiF-6DtGt6SqhAf6xqnAt4hsMDfvbYItnkFjvKVz17bUimAutlkz9dXTxc3wbSOQmDRHWrxOhIGLVIgOZAqtUoqJ5QBjb6BtqlGRpYI4zKdjCBBhwmAErGQ6liXFBCGYosNyqqEHcaNjSHTsQAXy7gwqY6M0UoKgx-QyE2G7Hhm2nzSy2XkSDMIgdwjkHsEhuyczD7vQhrXvgGRz6fI538hP2RHM9ByXBP0o0OXUHVNHqUZ6dqoEPts9yDOPyUk-pRI04ZMLcC7MJbFJ-Xb2Otuh5T0EkfR7n-Mfo-tUOV6CmeLxD4btHUHB2y5KbpDP5W_AM1s_js priority: 102 providerName: Directory of Open Access Journals
Title	The photosystem I supercomplex from a primordial green alga Ostreococcus tauri harbors three light-harvesting complex trimers
URI	https://www.ncbi.nlm.nih.gov/pubmed/36951548 https://search.proquest.com/docview/2790050810 https://pubmed.ncbi.nlm.nih.gov/PMC10097422 https://doaj.org/article/39f21376addb45ffa53e8de600170320
Volume	12
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9swDBaWnnYZNnTrsq2BBvTqJLZsWT5ufaDFHi2wFdjNkGQqCZDahh_ALvvvI-W4SIqddjFgWbYEfTRF2uRHxs4KZXVmKc5PZkUQSwmBTrUJbOZAK70E4Sgb-fpH-v2Xurgkmhw55sL4oH1rNvNy-zAvN2sfW1k_2MUYJ7a4-3YeUvZBHEWLCZugcbjno3v9m6JQhtmQjJfidrmArxsHc4WeCJXnExKtioQK_uztRJ6w_19W5tNgyb3d5-ole7EzG_mnYXqv2DMoj9kfxJjX66qrBj5mfsPbvobGh4nDb06pI1zzukE8SA62fEVRNpzSN_gtZYlUqA5t3_KOyIX4WjcoEXiG-ALfeooRbPNEHOWKj4_tqCJA075m91eXP8-vg105hcCiVdThcSkMLk6BPoJUqVVSOaEMaDQRtE01OmaJMC7TyRIStJsAKB8LPR7rkgLCULxhR2VVwlvGjY0h07EAF8u4MKmOjNFKCoMDSHRRpuxsXNq8HlgzcvQ2CIzcg5F7MKbsMy37YxeiuvYNVbPKd4DnInNRiGoQFbGJE-d0IkAVID3zj4iWU_ZxBC3HV4P-d-gSqr7NozQjehsVYp-TAcTHoUYhmDJ1AO_BXA6voDR6-u1R-t79_63v2XMqW0-xbJH4wI66podTNmmLfoZm_c2Xmf80MPNy_RdHtQFv
link.rule.ids	230,315,729,782,786,866,887,2106,27933,27934,53800,53802
linkProvider	National Library of Medicine
linkToHtml	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj5swELa620N76UN9pU9X2isJYDDm2G53lVWz20rdSr0h24yTSFlABKRe-t87Y8IqqXraCxLYYMvfeDwjZr5h7KRUVueW4vxkXgaJlBDoTJvA5g600iEIR9nI8x_Z1S_15YxocuSYC-OD9q1ZT6vNzbRar3xsZXNjZ2Oc2Oz75WlE2QdJHM-O2H3csGG456V7DZyhWEb5kI6X4YE5g8XawVShL0IF-oREuyKlkj97Z5Gn7P-fnflvuOTe-XP--K4zf8Ie7SxO_mlof8ruQfWM_UHx4M2q7uqByplf8G3fQOsjzOE3p6wTrnnTIpQkQhu-pAAdTpkf_BslmNSoSW2_5R3xEvGVblGY8A5FA_jGs5PgM8_hUS35-NmOigm02-fs5_nZ9ek82FViCCwaVB1eQ2FwVUt0L6TKrJLKCWVAo3WhbabRp0uFcblOQ0jR5AKgVC50lqxLS4gi8YIdV3UFrxg3NoFcJwJcIpPSZDo2RispDA4g0buZsJMRk6IZCDcKdFQIxcKjWHgUJ-wz4XXbhViy_YO6XRa7FS9E7uIINSjqcJOkzulUgCpBetIgEYcT9nFEu8BdRb9KdAV1vy3iLCdmHBVhn5cD-rdDjdIzYepALg7mctiC4uCZu0f4X9_91Q_swfz6clEsLq6-vmEPY7S5KCQuFm_Zcdf28I4dbcv-vd8QfwGUsRUz
linkToPdf	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Lb9QwELZokRAXHuLR5WmkXrN5OA-HG7RdtaKUSoDELbKd8e5K2yTKQ-LCf2fG2VS7iBNcIsVxYsvzeTyjzHzD2HEpjcoNxfmleenFaQqeypT2TG5BSRWAsJSNfP41u_ohT8-IJuf9lAvjgvaNXs-rzc28Wq9cbGVzY_wpTsy__nwSUvZBHEV-U1r_gN3FTRtEO56608IZQjPMx5S8DA9NHy7XFuYS_REq0idStC0SKvuzcx452v6_2Zp_hkzunEGLh_8z-0fswdby5B_GPo_ZHaiesF8IE96s6r4eKZ35Be-GBloXaQ4_OWWfcMWbFkVKUNrwJQXqcMoA4V8o0aRGjWqGjvfET8RXqkVQ4R1CBPjGsZRgm-PyqJZ8-mxPRQXa7in7vjj7dnLubSsyeAYNqx6vgdC4siW6GanMjEylFVKDQitDmUyhb5cIbXOVBJCg6QVAKV3oNBmblBCG4hk7rOoKjhjXJoZcxQJsnMalzlSktZKp0DhAil7OjB1PcimakXijQIeFJFk4SRZOkjP2kWR224XYsl1D3S6L7aoXIrdRiJoUdbmOE2tVIkCWkDryIBEFM_ZukniBu4t-magK6qEroiwnhhwZYp_nIwJuh5oQNGNyDxt7c9l_gpBwDN4TBF78-6tv2b3r00VxeXH16SW7H6HpRZFxkXjFDvt2gNfsoCuHN25P_AYn-hez
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+photosystem+I+supercomplex+from+a+primordial+green+alga+Ostreococcus+tauri+harbors+three+light-harvesting+complex+trimers&rft.jtitle=eLife&rft.au=Ishii%2C+Asako&rft.au=Shan%2C+Jianyu&rft.au=Sheng%2C+Xin&rft.au=Kim%2C+Eunchul&rft.date=2023-03-23&rft.eissn=2050-084X&rft.volume=12&rft_id=info:doi/10.7554%2FeLife.84488&rft.externalDBID=NO_FULL_TEXT
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2050-084X&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2050-084X&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2050-084X&client=summon