Protein shift and antigenic variation in the S-layer of Campylobacter fetus subsp. venerealis during bovine infection accompanied by genomic rearrangement of sapA homologs

• Published in: Journal of Bacteriology Vol. 177; no. 8; pp. 1976 - 1980
• Main Authors: Garcia, M.M. (Agriculture and Agri-Food Canada, Nepean, Ontario, Canada.), Lutze-Wallace, C, Denes, A.S, Eaglesome, M.D, Holst, E, Blaser, M.J
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01-04-1995
• Subjects: ADN; Animal reproduction; Animals; Antibodies, Monoclonal; ANTIGENE; Antigenic Variation; ANTIGENOS; Antigens, Bacterial - genetics; BACTERIA; Bacterial Proteins - genetics; Bacterial Proteins - immunology; Bacteriology; BOVIN; CAMPYLOBACTER; Campylobacter fetus - genetics; Campylobacter fetus - immunology; Campylobacter fetus - isolation & purification; Campylobacter Infections - microbiology; Campylobacter Infections - veterinary; Cattle; Cattle Diseases - microbiology; DNA, Bacterial - genetics; EXPERIMENTACION IN VIVO; EXPERIMENTATION IN VIVO; Female; GANADO BOVINO; GENE; Gene Rearrangement; GENES; Genes, Bacterial; GENISSE; Humans; INFECCION EXPERIMENTAL; INFECTION EXPERIMENTALE; Infections; Membrane Glycoproteins; Microscopy, Immunoelectron; NOVILLA; PROTEINAS; PROTEINE; UTERO; UTERUS; VAGIN; VAGINA; Vaginosis, Bacterial - microbiology; Vaginosis, Bacterial - veterinary; VARIACION GENETICA; VARIATION GENETIQUE
• ISSN: 0021-9193; 1098-5530; 1067-8832
• DOI: 10.1128/jb.177.8.1976-1980.1995

Campylobacter fetus subsp. venerealis isolated from a case of human vaginosis was inoculated into the uterus of a C. fetus-negative heifer. Isolates obtained weekly from the vaginal mucus exhibited variations in high-molecular-mass-protein profiles from that of the original inoculum, which had a dominant 110-kDa S-layer protein. Immunoblots of the weekly isolates with monoclonal antibody probes against the 110-kDa S-layer protein and other C. fetus S-layer proteins demonstrated antigenic shifts. Genomic digests of the isolates probed with a 75-mer oligonucleotide of the conserved sapA region also indicated that antigenic variation of the S-layer is accompanied by DNA rearrangement