En route to personalized medicine: uncovering distinct IgE reactivity pattern to house dust mite components in Brazilian and Austrian allergic patients

• Published in: Clinical and translational allergy Vol. 11; no. 1; pp. e12004 - n/a
• Main Authors: Pinheiro, Carina S., Silva, Eduardo S., de Andrade Belitardo, Emília M. M., Pacheco, Luis G. C., Aguiar, Eric R. G. R., Alcantara‐Neves, Neuza M., Gadermaier, Gabriele, Ferreira, Fatima
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01-03-2021; John Wiley and Sons Inc; Wiley
• Subjects: Allergens; Allergies; allergy; Letter to the Editor; mites; molecular diagnosis; Precision medicine; Brazil; Austria; allergy; mites; allergens; molecular diagnosis
• ISSN: 2045-7022; 2045-7022
• DOI: 10.1002/clt2.12004

Aim
Molecular sensitization profile analyses of allergic individuals to the house dust mites (HDM) Blomia tropicalis and Dermatophagoides pteronyssinus from Brazil and Austria, in the attempt to comprehend the individual contribution of the molecular components in the diagnosis of HDM allergy.
Methodology
These analyses were made using a new in vitro multiplex allergen assay which allows simultaneous measurement of specific IgE against the whole allergen extract as well its components.
Results and Conclusion
The data showed that in Brazil the inclusion of the molecular components Blo t 5 and/or Blo t 21 major allergens and Blo t 2 can increase the sensitivity and specificity of the assay for the diagnosis of allergy to B. tropicalis, using matrix‐based methodologies. Also we highlighted, for the first time, the importance of Blo t 2 analysis for a sensitive diagnosis, since some individuals were sensitized only to this molecular component. Regarding the sensitization profile of individuals sensitized to D. pteronyssinus, we point out the importance of analyzing the molecular components Der p23 and Der p 7, in addition to Der p 1 and Der p 2 for an accurate diagnosis based on matrices.