Super-resolution Microscopy Approaches for Live Cell Imaging

By delivering optical images with spatial resolutions below the diffraction limit, several super-resolution fluorescence microscopy techniques opened new opportunities to study biological structures with details approaching molecular structure sizes. They have now become methods of choice for imagin...

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Bibliographic Details
Published in:	Biophysical journal Vol. 107; no. 8; pp. 1777 - 1784
Main Authors:	Godin, Antoine G., Lounis, Brahim, Cognet, Laurent
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 21-10-2014 Biophysical Society The Biophysical Society
Subjects:	Biological Physics Biophysical Review Cells Diffraction Fluorescence Imaging, Three-Dimensional - methods Limit of Detection Microscopy, Fluorescence - methods Molecular structure Physics Proteins Single-Cell Analysis - methods nanoscopy single molecule detection localization microscopy Running title: Live cell super-resolution imaging (up to 6): super-resolution microscopy fluorescence microscopy live-cell imaging
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Summary:	By delivering optical images with spatial resolutions below the diffraction limit, several super-resolution fluorescence microscopy techniques opened new opportunities to study biological structures with details approaching molecular structure sizes. They have now become methods of choice for imaging proteins and their nanoscale dynamic organizations in live cells. In this mini-review, we describe and compare the main far-field super-resolution approaches that allow studying endogenous or overexpressed proteins in live cells.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1
ISSN:	0006-3495 1542-0086
DOI:	10.1016/j.bpj.2014.08.028