A simple, sensitive and non-destructive technique for characterizing bovine dental enamel erosion： attenuated total reflection Fourier transform infrared spectroscopy

• Published in: International journal of oral science Vol. 8; no. 1; pp. 54 - 60
• Main Authors: Kim, In-Hye, Son, Jun Sik, Min, Bong Ki, Kim, Young Kyoung, Kim, Kyo-Han, Kwon, Tae-Yub
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30-03-2016; Springer Nature B.V; Department of Dental Science, Graduate School, Kyungpook National University, Daegu, Korea%Korea Textile Development Institute, Daegu, Korea%Center for Research Facilities, Yeungnam University, Gyeongsan, Korea%Department of Conservative Dentistry, School of Dentistry, Kyungpook National University, Daegu, Korea%Department of Dental Biomaterials, School of Dentistry, Kyungpook National University, Daegu, Korea; Nature Publishing Group
• Subjects: Animals; ATR-FTIR; Beverages; Cattle; Dentistry; Hardness; Hydrogen-Ion Concentration; In Vitro Techniques; Medicine; Oral and Maxillofacial Surgery; Original; original-article; Orthopedics; Sensitivity and Specificity; Spectroscopy, Fourier Transform Infrared - methods; Surface Properties; Surgical Orthopedics; Tooth Erosion - pathology; 侵蚀程度; 傅立叶变换红外光谱; 敏感技术; 牙釉质; 表面光滑; 衰减全反射; 非破坏性; enamel erosion; Fourier transform infrared spectroscopy; microhardness; sensitivity; acidic beverage
• ISSN: 1674-2818; 2049-3169
• DOI: 10.1038/ijos.2015.58

Although many techniques are available to assess enamel erosion in vitro, a simple, non-destructive method with sufficient sensitivity for quantifying dental erosion is required. This study characterized the bovine dental enamel erosion induced by various acidic beverages in vitro using attenuated total reflection Fourier transform infrared （ATR-FTIR） spectroscopy. Deionized water （control） and 10 acidic beverages were selected to study erosion, and the pH and neutralizable acidity were measured. Bovine anterior teeth （110） were polished with up to 1 200-grit silicon carbide paper to produce flat enamel surfaces, which were then immersed in 20 mL of the beverages for 30 min at 37 ℃. The degree of erosion was evaluated using ATR-FTIR spectroscopy and Vickers＇ microhardness measurements. The spectra obtained were interpreted in two ways that focused on the ~1, ~3 phosphate contour： the ratio of the height amplitude of ~3 P04 to that of/11 P04 （Method 1） and the shift of the v3 P04 peak to a higher wavenumber （Method 2）. The percentage changes in microhardness after the erosion treatments were primarily affected by the pH of the immersion media. Regression analyses revealed highly significant correlations between the surface hardness change and the degree of erosion, as detected by ATR-FTIR spectroscopy （P〈0.001）. Method 1 was the most sensitive to these changes, followed by surface hardness change measurements and Method 2. This study suggests that ATR- FTIR spectroscopy is potentially advantageous over the microhardness test as a simple, non-destructive, sensitive technique for the quantification of enamel erosion.