Minichromosome of simian virus 40: presence of histone HI

Minichromosome of simian virus 40: presence of histone HI

In contrast to conclusions of previous studies /I–3/ claiming the absence of histone HI from the SV40 and polyoma viral minichromosomes we have found that a preparation of purified SV40 minichromosomes does contain histone HI. The content of HI in relation to other four histones in the SV40 minichro...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research Vol. 3; no. 8; pp. 2101 - 2114
Main Authors: Varshavsky, A.J., Bakayev, V.V., Chumackov, P.M., Georgiev, G.P.
Format: Journal Article
Language:English
Published: England Oxford University Press 01-08-1976
Subjects:
Cell Fractionation
Chromosomes - analysis
Chromosomes - ultrastructure
Histones - analysis
Nucleoproteins - analysis
Osmolar Concentration
Simian virus 40
Simian virus 40 - analysis
Simian virus 40 - ultrastructure
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In contrast to conclusions of previous studies /I–3/ claiming the absence of histone HI from the SV40 and polyoma viral minichromosomes we have found that a preparation of purified SV40 minichromosomes does contain histone HI. The content of HI in relation to other four histones in the SV40 minichromosomes is close to that in the cellular chromatin. Histone HI in the isolated SV40 minichromosomes is bound apparently to internucleosomal DNA stretches as was shown already for HI in the cellular chromatin /4/. In addition it was found that more than 90% of the purified SV40 minichromosomes migrated as a single discrete deoxyribonucleoprotein band upon agarose gel electrophoresis.
Bibliography:istex:89E89138E24E3EFAA6D2B728DD7AE024B548E59A
ark:/67375/HXZ-WBHFZGSK-9
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/3.8.2101