The proteome of frozen-thawed pig spermatozoa is dependent on the ejaculate fraction source

• Published in: Scientific reports Vol. 9; no. 1; p. 705
• Main Authors: Pérez-Patiño, Cristina, Li, Junwei, Barranco, Isabel, Martínez, Emilio A., Rodriguez-Martínez, Heriberto, Roca, Jordi, Parrilla, Inmaculada
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24-01-2019; Nature Publishing Group
• Subjects: 13; 13/31; 631/337/475; 631/80; 82; 82/16; 82/29; 82/58; 82/75; 82/80; Apoptosis; Cryopreservation; DNA fragmentation; Experiments; Fertility; Humanities and Social Sciences; Lipid peroxidation; multidisciplinary; Peroxidation; Proteomes; Science; Science (multidisciplinary); Sperm
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-36624-5

The preservation of sperm functional parameters and fertility post-cryopreservation largely varies in the porcine, a species with a fractionated ejaculate. Although intrinsic individual differences have primarily been linked to this variation, differences in protein abundance among frozen-thawed (FT)-spermatozoa are far more relevant. This study, performed in two experiments, looked for proteomic quantitative differences between FT-sperm samples differing in post-thaw viability, motility, apoptosis, membrane lipid peroxidation and nuclear DNA fragmentation. The spermatozoa were either derived from the sperm-rich ejaculate fraction (SRF) or the entire ejaculate (Experiment 1) or from the first 10 mL of the SRF, the remaining SRF and the post-SRF (Experiment 2). Quantitative sperm proteomic differences were analysed using a LC-ESI-MS/MS-based SWATH approach. In Experiment 1, FT-spermatozoa from the SRF showed better preservation parameters than those from the entire ejaculate, with 26
Sus scrofa
proteins with functional sperm relevance showing relative quantitative differences (FC ≥ 1.5) between sperm sources. In Experiment 2, FT-spermatozoa from the first 10 mL of the SRF and the remaining SRF were qualitatively better than those from the post-SRF, and 187 proteins showed relative quantitative differences among the three ejaculate sources. The results indicate that quantitative proteome differences are linked to sperm cryosurvival.