A rapid, automated flow cytometric method to measure activated degranulated platelets by density determination

• Published in: Thrombosis and haemostasis Vol. 89; no. 6; p. 1004
• Main Authors: Chapman, E Sabrinah, Sorette, Martin, Hetherington, Edward, Zelmanovic, David, Kling, Gail, Dugailliez, Jacques, Pujol-Moix, Nuria, Okrongly, David
• Format: Journal Article
• Language: English
• Published: Germany 01-06-2003
• Subjects: Automation; Calibration; Cell Degranulation; Flow Cytometry - instrumentation; Flow Cytometry - methods; Humans; P-Selectin - analysis; Platelet Activation; Platelet Function Tests - instrumentation; Platelet Function Tests - methods; Thrombin
• ISSN: 0340-6245
• DOI: 10.1055/s-0037-1613402

Platelet activation is reported to correlate with acute coronary syndromes. A platelet analysis method on the ADVIA 120 Hematology System provides rapid analysis of platelet density, reported as mean platelet component (MPC) concentration, utilizes routine hematology specimens, requires no pre-treatment, and thirty seconds to generate results. Sub-populations of platelets separated by density gradients showed excellent correlation with the ADVIA 120 MPC parameter (r = 0.997). Platelet activation induced by thrombin treatment resulted in a shift of platelets into the lowest density fraction (d</=1.068 g/mL) with a corresponding reduction in MPC from 24.7 to 20.6 g/dL, N = 4 subjects (p < 0.004). There was also excellent correlation between expression of CD62P measured by fluorescence flow cytometry and the ADVIA 120 Hematology System MPC values (r = 0.85). These results indicate that the ADVIA 120 MPC parameter may be a useful new test for assessing activated platelets and platelet density.