Application of hydrogenase in biotechnological conversions

• Published in: Biochimie Vol. 68; no. 1; p. 201
• Main Authors: van Berkel-Arts, A, Dekker, M, van Dijk, C, Grande, H J, Hagen, W R, Hilhorst, R, Krüse-Wolters, M, Laane, C, Veeger, C
• Format: Journal Article
• Language: English
• Published: France 01-01-1986
• Subjects: Catalysis; Chemical Phenomena; Chemistry; Electrochemistry; Electrodes; Electron Transport; Hydrogen - metabolism; Hydrogen-Ion Concentration; Hydrogenase - metabolism; Kinetics; Micelles; Oxidation-Reduction; Oxidoreductases - metabolism; Photochemistry; Prednisone - metabolism; Progesterone - metabolism; Protons; Technology
• ISSN: 0300-9084
• DOI: 10.1016/S0300-9084(86)81084-7

Evidence will be presented in this review article that the application of hydrogenase has large biotechnological possibilities. Our investigations show: Fast reaction of hydrogenase at an electrode surface to reduce H+; Photochemical production of H2 by hydrogenase by photosensitized Ru-complexes dissolved in reversed micellar membranes and vectorial H+ transport through the membrane to the water phase; The production of fine chemicals in reversed micelles by a system containing specific enzymes, hydrogenase and H2. The rules to obtain maximal conversion rates with this system will be presented.