Antifungal Streptomyces spp. Associated with the Infructescences of Protea spp. in South Africa

• Published in: Frontiers in microbiology Vol. 7; p. 1657
• Main Authors: Human, Zander R, Moon, Kyuho, Bae, Munhyung, de Beer, Z Wilhelm, Cha, Sangwon, Wingfield, Michael J, Slippers, Bernard, Oh, Dong-Chan, Venter, Stephanus N
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 02-11-2016
• Subjects: Antifungal; Candida albicans; Fungichromin; Microbiology; Protea; Sporothrix; Streptomyces; Candida albicans; antifungal; infructescence; fungichromin; Sporothrix; Streptomyces; actiphenol; Protea
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2016.01657

Common saprophytic fungi are seldom present in
infructescences, which is strange given the abundance of mainly dead plant tissue in this moist protected environment. We hypothesized that the absence of common saprophytic fungi in
infructescences could be due to a special symbiosis where the presence of microbes producing antifungal compounds protect the infructescence. Using a culture based survey, employing selective media and
antifungal assays, we isolated antibiotic producing actinomycetes from infructescences of
and
.
from two geographically separated areas. Isolates were grouped into three different morphological groups and appeared to be common in the
spp. examined in this study. The three groups were supported in 16S rRNA and multi-locus gene trees and were identified as potentially novel
spp. All of the groups had antifungal activity
.
sp. Group 1 had inhibitory activity against all tested fungi and the active compound produced by this species was identified as fungichromin.
spp. Groups 2 and 3 had lower inhibition against all tested fungi, while Group 3 showed limited inhibition against
and
isolates. The active compound for Group 2 was also identified as fungichromin even though its production level was much lower than Group 1. The antifungal activity of Group 3 was linked to actiphenol. The observed antifungal activity of the isolated actinomycetes could contribute to protection of the plant material against common saprophytic fungi, as fungichromin was also detected in extracts of the infructescence. The results of this study suggest that the antifungal
spp. could play an important role in defining the microbial population associated with
infructescences.