Decrease of oxidative phosphorylation system function in severe septic patients

• Published in: Journal of critical care Vol. 30; no. 5; pp. 935 - 939
• Main Authors: Lorente, Leonardo, MD, PhD, Martín, María M., MD, PhD, López-Gallardo, Ester, PhD, Blanquer, José, MD, PhD, Solé-Violán, Jordi, MD, PhD, Labarta, Lorenzo, MD, Díaz, César, MD, Jiménez, Alejandro, PhD, Montoya, Julio, PhD, Ruiz-Pesini, Eduardo, PhD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-10-2015; Elsevier Limited
• Subjects: Adult; Age; Aged; Biomarkers - metabolism; Blood; Blood platelets; Blood Platelets - metabolism; Cardiovascular disease; Chronic obstructive pulmonary disease; Critical Care; Cytochrome c oxidase; Diabetes; Family medical history; Female; HIV; Hospitals; Human immunodeficiency virus; Humans; Hypotheses; Hypoxia; Infections; Intensive Care Units; Male; Middle Aged; Mitochondria; Mitochondria - metabolism; Mitochondrial Diseases - metabolism; Mitochondrial Diseases - mortality; Mortality; Oxidative Phosphorylation; OXPHOS; Phosphorylation; Physiology; Prospective Studies; Sepsis; Sepsis - metabolism; Sepsis - mortality; Studies; Cytochrome c oxidase; OXPHOS; Sepsis; Mitochondria; Mortality
• ISSN: 0883-9441; 1557-8615
• DOI: 10.1016/j.jcrc.2015.05.031

Abstract Objective The comparison of oxidative phosphorylation system capacities between septic patients and control subjects has been scarcely analyzed and only in studies with small sample size (fewer than 40 septic patients and 40 controls). Thus, the objective of this study was to compare platelet respiratory complex IV (CIV) activity between severe septic patients and healthy individuals in a larger series (including 198 severe septic patients and 96 healthy controls). Methods A prospective, multicenter, observational study was carried out in 6 Spanish intensive care units. We obtained blood samples from 198 severe septic patients at day 1, 4, and 8 of the severe sepsis diagnosis and 96 sex- and age-matched healthy control individuals and determined platelet CIV-specific activity. The end point of the study was 30-day mortality. Results Control individuals showed higher platelet CIV-specific activity ( P < .001) than surviving (n = 130) or nonsurviving (n = 68) severe septic patients at day 1, 4, and 8 of severe sepsis diagnosis. Conclusions The major finding of our work, involving the largest series to date of severe septic patients with data on oxidative phosphorylation system capacity, was that surviving and nonsurviving septic patients showed lower platelet CIV-specific activity during the first week of sepsis than healthy controls.