Characterization of 15 nuclear microsatellite markers for Afzelia africana (Fabaceae) and related species

• Published in: Applications in plant sciences Vol. 7; no. 5; pp. e01249 - n/a
• Main Authors: Houehanou, Thierry D., Prinz, Kathleen, Hellwig, Frank
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01-05-2019; John Wiley and Sons Inc; Wiley
• Subjects: Afzelia africana; Archives & records; conservation genetics; Deoxyribonucleic acid; DNA; Fabaceae; Gene polymorphism; Genetic diversity; Genetic markers; Heterozygosity; Microsatellites; Plant sciences; Polymorphism; Population; Population genetics; Population studies; Primer Note; Primer Notes; Scholarships & fellowships; simple sequence repeat (SSR); Species; sustainable management; West Africa; Gabon; Benin; Cameroon; United States > US; West Africa; California; Kenya; Germany; sustainable management; simple sequence repeat (SSR); West Africa; Afzelia africana; Fabaceae; conservation genetics
• ISSN: 2168-0450; 2168-0450
• DOI: 10.1002/aps3.1249

Premise
Afzelia africana (Fabaceae) is a valuable, internationally vulnerable tree species in tropical Africa. The development of specific simple sequence repeat (SSR) loci is necessary for population genetic studies in this tree species and its closest relatives.
Methods And Results
Fifteen new polymorphic microsatellite markers were developed for A. africana using Illumina next‐generation sequencing. We tested the polymorphism of the 15 loci in three populations in Benin, West Africa. The number of expressed alleles per locus varied from one to 12. The levels of observed and expected heterozygosity ranged from 0.100 to 1.000 and from 0.095 to 0.882, respectively. Most markers successfully amplified in the closely related species A. quanzensis and A. bipindensis, but less so in A. bipindensis.
Conclusions
Because of their cross‐amplification ability, these newly developed loci will serve as useful tools for future molecular analyses on A. africana and related species.