Nod1 Participates in the Innate Immune Response to Pseudomonas aeruginosa

The mammalian innate immune system recognizes pathogen-associated molecular patterns through pathogen recognition receptors. Nod1 has been described recently as a cytosolic receptor that detects specifically diaminopimelate-containing muropeptides from Gram-negative bacteria peptidoglycan. In the pr...

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Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 280; no. 44; pp. 36714 - 36718
Main Authors:	Travassos, Leonardo H., Carneiro, Leticia A.M., Girardin, Stephen E., Boneca, Ivo G., Lemos, Ramon, Bozza, Marcelo T., Domingues, Regina C.P., Coyle, Anthony J., Bertin, John, Philpott, Dana J., Plotkowski, Maria Cristina
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 04-11-2005 American Society for Biochemistry and Molecular Biology
Subjects:	Adaptor Proteins, Signal Transducing - genetics Adaptor Proteins, Signal Transducing - physiology Animals Apoptosis Cytokines - metabolism Epithelial Cells - cytology Epithelial Cells - immunology Epithelial Cells - metabolism Genes, Dominant Humans Immunity, Innate Kinetics Mice Mice, Knockout - microbiology NF-kappa B - metabolism Nod1 Signaling Adaptor Protein Peptidoglycan - immunology Pseudomonas aeruginosa - pathogenicity Pseudomonas Infections - immunology Pseudomonas Infections - metabolism
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Summary:	The mammalian innate immune system recognizes pathogen-associated molecular patterns through pathogen recognition receptors. Nod1 has been described recently as a cytosolic receptor that detects specifically diaminopimelate-containing muropeptides from Gram-negative bacteria peptidoglycan. In the present study we investigated the potential role of Nod1 in the innate immune response against the opportunistic pathogen Pseudomonas aeruginosa. We demonstrate that Nod1 detects the P. aeruginosa peptidoglycan leading to NF-κB activation and that this activity is diminished in epithelial cells expressing a dominant-negative Nod1 construct or in mouse embryonic fibroblasts from Nod1 knock-out mice infected with P. aeruginosa. Finally, we demonstrate that the cytokine secretion kinetics and bacterial killing are altered in Nod1-deficient cells infected with P. aeruginosa in the early stages of infection.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0021-9258 1083-351X
DOI:	10.1074/jbc.M501649200