Expression of bacterial phosphite dehydrogenase confers phosphite availability in a unicellular red alga Cyanidioschyzon merolae
Microalgae are promising cell factories for producing value-added products. Large-scale microalgal cultivation suffers from invasion by contaminating microorganisms. Since most contaminating organisms cannot utilize phosphite as a unique phosphorus source, phosphite-utilizing ability may provide a g...
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| Published in: | Journal of general and applied microbiology Vol. 69; no. 5; pp. 287 - 291 |
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| Abstract | Microalgae are promising cell factories for producing value-added products. Large-scale microalgal cultivation suffers from invasion by contaminating microorganisms. Since most contaminating organisms cannot utilize phosphite as a unique phosphorus source, phosphite-utilizing ability may provide a growth advantage against contaminating organisms and solve this problem. Studies showed that microorganisms, typically unable to metabolize phosphite, can utilize phosphite by expressing exogenous phosphite dehydrogenase. Here, we constructed Cyanidioschyzon merolae strains introduced with the phosphite dehydrogenase gene, ptxD, from Ralstonia sp. 4506. The ptxD-introduced strains grew in a phosphite-dependent manner, with the phosphite-related growth rate almost matching that with phosphate as sole phosphorus source. |
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| AbstractList | Microalgae are promising cell factories for producing value-added products. Large-scale microalgal cultivation suffers from invasion by contaminating microorganisms. Since most contaminating organisms cannot utilize phosphite as a unique phosphorus source, phosphite-utilizing ability may provide a growth advantage against contaminating organisms and solve this problem. Studies showed that microorganisms, typically unable to metabolize phosphite, can utilize phosphite by expressing exogenous phosphite dehydrogenase. Here, we constructed Cyanidioschyzon merolae strains introduced with the phosphite dehydrogenase gene, ptxD, from Ralstoniasp. 4506. The ptxD-introduced strains grew in a phosphite-dependent manner, with the phosphite-related growth rate almost matching that with phosphate as sole phosphorus source. Microalgae are promising cell factories for producing value-added products. Large-scale microalgal cultivation suffers from invasion by contaminating microorganisms. Since most contaminating organisms cannot utilize phosphite as a unique phosphorus source, phosphite-utilizing ability may provide a growth advantage against contaminating organisms and solve this problem. Studies showed that microorganisms, typically unable to metabolize phosphite, can utilize phosphite by expressing exogenous phosphite dehydrogenase. Here, we constructed Cyanidioschyzon merolae strains introduced with the phosphite dehydrogenase gene, ptxD, from Ralstonia sp. 4506. The ptxD-introduced strains grew in a phosphite-dependent manner, with the phosphite-related growth rate almost matching that with phosphate as sole phosphorus source. |
| ArticleNumber | 2023.08.002 |
| Author | Kuroda, Akio Tanaka, Kan Hirota, Ryuichi Kobayashi, Ikki Imamura, Sousuke |
| Author_xml | – sequence: 1 fullname: Kobayashi, Ikki organization: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology – sequence: 2 fullname: Imamura, Sousuke organization: Space Environment and Energy Laboratories, Nippon Telegraph and Telephone Corporation – sequence: 3 fullname: Hirota, Ryuichi organization: Unit of Biotechnology, Graduate School of Integrated Sciences for Life, Hiroshima University – sequence: 4 fullname: Kuroda, Akio organization: Unit of Biotechnology, Graduate School of Integrated Sciences for Life, Hiroshima University – sequence: 5 fullname: Tanaka, Kan organization: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/37587047$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1128/AEM.71.1.290-296.2005 10.1073/pnas.0400664101 10.1016/j.biortech.2011.05.033 10.1073/pnas.0708205105 10.1093/pcp/pcab052 10.2323/jgam.2021.11.001 10.2323/jgam.61.211 10.1093/pcp/pcq043 10.1016/j.bbrep.2017.01.010 10.1186/1471-2164-14-753 10.1016/j.febslet.2013.08.031 10.1021/es0401038 10.1111/tpj.14473 10.1126/science.aaf6159 10.1093/pcp/pch087 10.1007/s00253-019-10258-7 10.1146/annurev.micro.61.080706.093357 10.1016/j.rser.2009.07.020 10.2323/jgam.2017.02.002 10.1104/pp.104.053991 10.1038/nature02398 10.1093/pcp/pcy156 10.1007/s00203-002-0402-x 10.1007/s11356-023-27644-4 10.1128/jb.80.2.237-241.1960 10.1111/pbi.12564 10.1016/j.jbiosc.2011.11.027 10.1016/j.biortech.2012.10.158 10.1021/acssynbio.8b00199 10.1080/21655979.2017.1377867 10.1128/JB.180.21.5547-5558.1998 10.1105/tpc.106.045427 10.3389/fbioe.2021.647176 10.1038/srep44748 10.1002/bit.25357 10.1186/1741-7007-5-28 |
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| Keywords | Cyanidioschyzon merolae Microalgae Phosphorus metabolism Phosphite dehydrogenase Microalgal cultivation Phosphite |
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| SubjectTerms | Algae Aquatic microorganisms Contamination Cyanidioschyzon merolae Dehydrogenase Microalgae Microalgal cultivation Microorganisms NADH, NADPH Oxidoreductases - genetics Phosphite Phosphite dehydrogenase Phosphites - metabolism Phosphorus Phosphorus metabolism Rhodophyta - genetics Strains (organisms) |
| Title | Expression of bacterial phosphite dehydrogenase confers phosphite availability in a unicellular red alga Cyanidioschyzon merolae |
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