The outer mucus layer hosts a distinct intestinal microbial niche

• Published in: Nature communications Vol. 6; no. 1; p. 8292
• Main Authors: Li, Hai, Limenitakis, Julien P., Fuhrer, Tobias, Geuking, Markus B., Lawson, Melissa A., Wyss, Madeleine, Brugiroux, Sandrine, Keller, Irene, Macpherson, Jamie A., Rupp, Sandra, Stolp, Bettina, Stein, Jens V., Stecher, Bärbel, Sauer, Uwe, McCoy, Kathy D., Macpherson, Andrew J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22-09-2015; Nature Publishing Group; Nature Pub. Group
• Subjects: 14/19; 14/35; 14/69; 631/158/855; 631/326/2565; 64/60; 692/4020/2741; 82/58; 96/31; Animals; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Gene Expression Regulation, Bacterial; Germ-Free Life; Humanities and Social Sciences; Intestinal Mucosa - microbiology; Iron - metabolism; Mice; Mice, Inbred C57BL; multidisciplinary; Multigene Family; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Science; Science (multidisciplinary); Transcriptome
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms9292

The overall composition of the mammalian intestinal microbiota varies between individuals: within each individual there are differences along the length of the intestinal tract related to host nutrition, intestinal motility and secretions. Mucus is a highly regenerative protective lubricant glycoprotein sheet secreted by host intestinal goblet cells; the inner mucus layer is nearly sterile. Here we show that the outer mucus of the large intestine forms a unique microbial niche with distinct communities, including bacteria without specialized mucolytic capability. Bacterial species present in the mucus show differential proliferation and resource utilization compared with the same species in the intestinal lumen, with high recovery of bioavailable iron and consumption of epithelial-derived carbon sources according to their genome-encoded metabolic repertoire. Functional competition for existence in this intimate layer is likely to be a major determinant of microbiota composition and microbial molecular exchange with the host.
The inner layer of the mucus that covers our intestine is nearly sterile. Here, the authors show in mice that the outer mucus layer constitutes a unique microbial niche hosting bacterial communities with distinct proliferation rates and resource utilization activities.