Effects of energetic restriction diet on butyrylcholinesterase in obese women from southern Brazil - A longitudinal study

• Published in: Archives of Endocrinology and Metabolism Vol. 61; no. 5; pp. 484 - 489
• Main Authors: Santos, Willian Dos, Tureck, Luciane Viater, Saliba, Louise Farah, Schenknecht, Caroline Schovanz, Scaraboto, Débora, Souza, Ricardo Lehtonen R, Furtado-Alle, Lupe
• Format: Journal Article
• Language: English
• Published: Brazil Sociedade Brasileira de Endocrinologia e Metabologia 01-09-2017; Brazilian Society of Endocrinology and Metabolism
• Subjects: Adult; BChE molecular forms; Brazil; Butyrylcholinesterase - metabolism; butyrylcholinesterase activity; Caloric Restriction; dietetic intervention; Energy Metabolism; Female; Humans; lipid metabolism; Longitudinal Studies; Middle Aged; Obesity; Obesity - diet therapy; Obesity - enzymology; Original; Phenotype; Regression Analysis
• ISSN: 2359-3997; 2359-4292; 2359-4292; 2359-3997
• DOI: 10.1590/2359-3997000000268

Butyrylcholinesterase (BChE) activity has been associated with obesity, lipid concentrations, and CHE2 locus phenotypes. This, the aim of this study was to evaluate the effects of an energetic restriction diet intervention on anthropometrical and biochemical variables and on absolute and relative BChE activity in CHE2 C5+ and CHE2 C5- individuals.
One hundred eleven premenopausal obese women from Southern Brazil participated in an energetic restriction diet intervention (deficit of 2500 kJ/day) for 8 weeks. Their anthropometric and biochemical parameters were evaluated before and after the intervention. Plasma BChE activity was measured, and BChE bands in plasma and CHE2 locus phenotypes were detected by electrophoresis.
The dietetic intervention decreased anthropometric and biochemical parameters as well as absolute BChE activity and relative activity of the G4 band. The CHE2 C5+ phenotype presented a different effect when compared with the CHE2 C5- phenotype. The CHE2 C5+ phenotype showed an effect in absolute BChE activity and in the relative activity of the G4 form, maintaining higher BChE activity regardless of the metabolic changes.
In our study, 8 weeks was not sufficient time to lower the body mass index to normal, but it was enough to significantly reduce the absolute BChE activity, which became similar to the levels in nonobese individuals. CHE2 C5+ individuals were resistant to the decrease in BChE activity compared to CHE2 C5- individuals. This shows that the diet did not affect the CHE2 and G4 fraction complex and that the products of the CHE2 locus in association with BChE have a role in energy metabolism, maintaining high levels of enzymatic activity even after dietary intervention.