Expression of Umbelopsis ramanniana DGAT2A in Seed Increases Oil in Soybean

• Published in: Plant physiology (Bethesda) Vol. 148; no. 1; pp. 89 - 96
• Main Authors: Lardizabal, Kathryn, Effertz, Roger, Levering, Charlene, Mai, Jennifer, Pedroso, M.C, Jury, Tom, Aasen, Eric, Gruys, Ken, Bennett, Kristen
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Biologists 01-09-2008; American Society of Plant Physiologists
• Subjects: Agriculture; Biochemical Processes and Macromolecular Structures; Biological and medical sciences; Developmental biology; Diacylglycerol O-Acyltransferase - genetics; Diacylglycerol O-Acyltransferase - metabolism; Diglycerides; Fundamental and applied biological sciences. Psychology; Genes, Fungal; Glycine max - genetics; Glycine max - growth & development; Glycine max - metabolism; Lipid bodies; Lipids; Mucorales - genetics; Oilseeds; Phenotype; Plant physiology and development; Plants; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Proteins; Seeds; Seeds - growth & development; Seeds - metabolism; Soybean Oil - biosynthesis; Soybeans; Transgenic plants; Grain legume; Seeds; Leguminosae; Plant physiology; Dicotyledones; Angiospermae; Spermatophyta; Soybean; Glycine max
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1104/pp.108.123042

Oilseeds are the main source of lipids used in both food and biofuels. The growing demand for vegetable oil has focused research toward increasing the amount of this valuable component in oilseed crops. Globally, soybean (Glycine max) is one of the most important oilseed crops grown, contributing about 30% of the vegetable oil used for food, feed, and industrial applications. Breeding efforts in soy have shown that multiple loci contribute to the final content of oil and protein stored in seeds. Genetically, the levels of these two storage products appear to be inversely correlated with an increase in oil coming at the expense of protein and vice versa. One way to overcome the linkage between oil and protein is to introduce a transgene that can specifically modulate one pathway without disrupting the other. We describe the first, to our knowledge, transgenic soy crop with increased oil that shows no major impact on protein content or yield. This was achieved by expressing a codon-optimized version of a diacylglycerol acyltransferase 2A from the soil fungus Umbelopsis (formerly Mortierella) ramanniana in soybean seed during development, resulting in an absolute increase in oil of 1.5% (by weight) in the mature seed.