Imaging inflammation in mouse colon using a rapid stage-scanning confocal fluorescence microscope

Imaging inflammation in mouse colon using a rapid stage-scanning confocal fluorescence microscope

Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue processing in comparison to histology. We present a rapid beam and stage-scanning confocal fluorescence microscope to image cellular and tissue feat...

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Published in:Journal of Biomedical Optics Vol. 17; no. 1; pp. 016006 - 016008
Main Authors: Saldua, Meagan A, Olsovsky, Cory A, Callaway, Evelyn S, Chapkin, Robert S, Maitland, Kristen C
Format: Journal Article
Language:English
Published: United States Society of Photo-Optical Instrumentation Engineers 01-01-2012
Subjects:
Animals
Beams (radiation)
chronic inflammation
Colitis - pathology
Colon
Colon - pathology
Confocal
Confocal microscopy
Disease Models, Animal
Equipment Design
Fluorescence
Histocytochemistry
Image contrast
Imaging
Inflammation - pathology
inflammatory bowel disease
Inflammatory Bowel Diseases - pathology
large area microscopy
Mice
Mice, Inbred C57BL
Microscopes
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Research Papers: Imaging
stage scanning
Online Access:Get full text
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Abstract Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue processing in comparison to histology. We present a rapid beam and stage-scanning confocal fluorescence microscope to image cellular and tissue features along the length of the entire excised mouse colon. The beam is scanned at by a polygon scanning mirror while the specimen is scanned in the orthogonal axis by a motorized translation stage with a maximum speed of . A single field of view image spanning the length of the mouse colon is acquired in 10 s. Z-projection images generated from axial image stacks allow high resolution imaging of the surface of non-flat specimens. In contrast to the uniform size, shape, and distribution of colon crypts in confocal images of normal colon, confocal images of chronic bowel inflammation exhibit heterogeneous tissue structure with localized severe crypt distortion.
AbstractList Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue processing in comparison to histology. We present a rapid beam and stage-scanning confocal fluorescence microscope to image cellular and tissue features along the length of the entire excised mouse colon. The beam is scanned at 8 , 333    lines / sec by a polygon scanning mirror while the specimen is scanned in the orthogonal axis by a motorized translation stage with a maximum speed of 7    mm / sec . A single 1 × 60   mm 2 field of view image spanning the length of the mouse colon is acquired in 10 s. Z-projection images generated from axial image stacks allow high resolution imaging of the surface of non-flat specimens. In contrast to the uniform size, shape, and distribution of colon crypts in confocal images of normal colon, confocal images of chronic bowel inflammation exhibit heterogeneous tissue structure with localized severe crypt distortion.
Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue processing in comparison to histology. We present a rapid beam and stage-scanning confocal fluorescence microscope to image cellular and tissue features along the length of the entire excised mouse colon. The beam is scanned at by a polygon scanning mirror while the specimen is scanned in the orthogonal axis by a motorized translation stage with a maximum speed of . A single field of view image spanning the length of the mouse colon is acquired in 10 s. Z-projection images generated from axial image stacks allow high resolution imaging of the surface of non-flat specimens. In contrast to the uniform size, shape, and distribution of colon crypts in confocal images of normal colon, confocal images of chronic bowel inflammation exhibit heterogeneous tissue structure with localized severe crypt distortion.
Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue processing in comparison to histology. We present a rapid beam and stage-scanning confocal fluorescence microscope to image cellular and tissue features along the length of the entire excised mouse colon. The beam is scanned at 8, 333 lines/sec by a polygon scanning mirror while the specimen is scanned in the orthogonal axis by a motorized translation stage with a maximum speed of 7 mm/sec. A single 1 x 60 mm super(2) field of view image spanning the length of the mouse colon is acquired in 10 s. Z-projection images generated from axial image stacks allow high resolution imaging of the surface of non-flat specimens. In contrast to the uniform size, shape, and distribution of colon crypts in confocal images of normal colon, confocal images of chronic bowel inflammation exhibit heterogeneous tissue structure with localized severe crypt distortion.
Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue processing in comparison to histology. We present a rapid beam and stage-scanning confocal fluorescence microscope to image cellular and tissue features along the length of the entire excised mouse colon. The beam is scanned at 8,333 lines/sec by a polygon scanning mirror while the specimen is scanned in the orthogonal axis by a motorized translation stage with a maximum speed of 7 mm/sec. A single 1 × 60 mm(2) field of view image spanning the length of the mouse colon is acquired in 10 s. Z-projection images generated from axial image stacks allow high resolution imaging of the surface of non-flat specimens. In contrast to the uniform size, shape, and distribution of colon crypts in confocal images of normal colon, confocal images of chronic bowel inflammation exhibit heterogeneous tissue structure with localized severe crypt distortion.
Author Saldua, Meagan A
Callaway, Evelyn S
Maitland, Kristen C
Chapkin, Robert S
Olsovsky, Cory A
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Snippet Large area confocal microscopy may provide fast, high-resolution image acquisition for evaluation of tissue in pre-clinical studies with reduced tissue...
SourceID pubmedcentral
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StartPage 016006
SubjectTerms Animals
Beams (radiation)
chronic inflammation
Colitis - pathology
Colon
Colon - pathology
Confocal
Confocal microscopy
Disease Models, Animal
Equipment Design
Fluorescence
Histocytochemistry
Image contrast
Imaging
Inflammation - pathology
inflammatory bowel disease
Inflammatory Bowel Diseases - pathology
large area microscopy
Mice
Mice, Inbred C57BL
Microscopes
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Research Papers: Imaging
stage scanning
Title Imaging inflammation in mouse colon using a rapid stage-scanning confocal fluorescence microscope
URI http://dx.doi.org/10.1117/1.JBO.17.1.016006
https://www.ncbi.nlm.nih.gov/pubmed/22352656
https://search.proquest.com/docview/1541437846
https://search.proquest.com/docview/1677911280
https://search.proquest.com/docview/923190503
https://pubmed.ncbi.nlm.nih.gov/PMC3380810
Volume 17
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