Developmental expression of the glucose dehydrogenase gene in Drosophila melanogaster

• Published in: Genetics (Austin) Vol. 124; no. 4; pp. 873 - 880
• Main Authors: Cox-Foster, D.L. (Pennsylvania State University, University Park, PA), Schonbaum, C.P, Murtha, M.T, Cavener, D.R
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Genetics Soc America 01-04-1990; Genetics Society of America
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; Animals; Biological and medical sciences; Blotting, Northern; Carbohydrate Dehydrogenases - genetics; CONTROL GENETICO; Diptera; DROSOPHILA; Drosophila melanogaster - enzymology; Drosophila melanogaster - genetics; Drosophila melanogaster - growth & development; Drosophilidae; Ecdysterone - biosynthesis; Ecdysterone - genetics; ENZYMIC ACTIVITY; Female; FENOTIPOS; Fundamental and applied biological sciences. Psychology; GENE; Gene expression; Gene Expression Regulation, Enzymologic; GENES; GENETIC CONTROL; GLUCOSA; GLUCOSE; Glucose Dehydrogenases - biosynthesis; Glucose Dehydrogenases - genetics; Investigations; LUTTE GENETIQUE; Male; Molecular and cellular biology; Molecular genetics; MUTANT; MUTANTES; MUTANTS; Nucleic Acid Hybridization; OXIDOREDUCTASES; OXIDORREDUCTASAS; OXYDOREDUCTASE; PHENOTYPE; PHENOTYPES; RNA, Messenger - analysis; Molecular hybridization; Enzyme; Insecta; In situ; Genital system; Gene expression; Ecdysone β; Drosophilidae; Glucose dehydrogenase; Arthropoda; Development; Invertebrata; Drosophila melanogaster; Metamorphosis; Diptera
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1093/genetics/124.4.873

The Gld gene of Drosophila melanogaster is transiently expressed during every stage of development. The temporal pattern of Gld expression is highly correlated with that of ecdysteroids. Exogeneous treatment of third instar larvae with 20-hydroxyecdysone induces the accumulation of Gld mRNA in the hypoderm and anterior spiracular gland cells. During metamorphosis Gld is expressed in a variety of tissues derived from the ectoderm. In the developing reproductive tract, Gld mRNA accumulates in the female spermathecae and oviduct and in the male ejaculatory duct and ejaculatory bulb. These four organs are derived from closely related cell lineages in the genital imaginal disc. Since the expression of Gld is not required for the development of these reproductive structures, this spatial pattern of expression is most likely a fortuitous consequence of a shared regulatory factor in this cell lineage. At the adult stage a high level of the Gld mRNA is only observed in the male ejaculatory duct.