In situ and laboratory bioassays with Chironomus riparius larvae to assess toxicity of metal contamination in rivers: the relative toxic effect of sediment versus water contamination

• Published in: Environmental toxicology and chemistry Vol. 26; no. 9; pp. 1968 - 1977
• Main Authors: Faria, M.S, Lopes, R.J, Nogueira, A.J.A, Soares, A.M.V.M
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Periodicals, Inc 01-09-2007; Blackwell Publishing Ltd
• Subjects: Acid mine drainage; Animals; aquatic insects; Bioassays; Bioavailability; Biological Assay - methods; Biological responses; body measurements; Chironomidae - anatomy & histology; Chironomidae - drug effects; Chironomus riparius; Contaminated sediments; Contamination; field experimentation; Freshwater; Geologic Sediments - chemistry; gold; head; Heavy metals; indicator species; Ingestion; Larva - anatomy & histology; Larva - drug effects; Larvae; Metal contamination; metals; Metals, Heavy - analysis; Metals, Heavy - toxicity; Microbiology; Mines; mining; Organic matter; particulates; Polyvinyl chloride; Portugal; river water; Rivers; Rivers - chemistry; sediment contamination; Sediment pollution; Soil Pollutants - toxicity; streams; Time Factors; Toxicity; toxicity testing; Water column; Water flow; Water Pollutants, Chemical - toxicity; Water pollution; Portugal
• ISSN: 0730-7268; 1552-8618
• DOI: 10.1897/06-435R1.1

We used bioassays employing head capsule width and body length increase of Chironomus riparius larvae as end points to evaluate metal contamination in streams. Bioassays were performed in situ near an abandoned Portuguese goldmine in the spring of 2003 and 2004. Bioassays also were performed under laboratory conditions with water and sediment collected from each stream to verify if laboratory bioassays could detect in situ toxicity and to evaluate the relative contribution of sediment and water to overall toxicity. We used field sediments with control water and control sediments with field water to discriminate between metal contamination in water and sediment. Field water with dry and sieved, organic matter–free, and nontreated sediments was used to determine the toxicity of heavy metals that enter the organism through ingested material. In both in situ and laboratory bioassays, body length increase was significantly inhibited by metal contamination, whereas head capsule width was not affected. Body length increase was more affected by contaminated sediment compared to contaminated water. The lowest‐effect level of heavy metals was observed in the dry and sieved sediment that prevented ingestion of sediment particles by larvae. These results suggest that body length increase of C. riparius larvae can be used to indicate the impact of metal contamination in rivers. Chironomus riparius larvae are more affected by heavy metals that enter the organism through ingested sediment than by heavy metals dissolved in the water column. Nevertheless, several factors, such as the particle size and organic matter of sediment, must be taken into account.