High throughput isolation of RNA from single-cells within an intact tissue for spatial and temporal sequencing a reality

Single-cell transcriptomics is essential for understanding biological variability among cells in a heterogenous population. Acquiring high-quality single-cell sequencing data from a tissue sample has multiple challenges including isolation of individual cells as well as amplification of the genetic...

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Bibliographic Details
Published in:	PloS one Vol. 18; no. 8; p. e0289279
Main Authors:	Stanley, John, Lohith, Akshar, Debiaso, Lucca, Wang, Kevan, Ton, Minh, Cui, Wenwu, Gu, Weiwei, Fu, Aihua, Pourmand, Nader
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 01-08-2023
Subjects:	Analysis Automation Bar codes Biology and life sciences Breast cancer Cell division Cells Data acquisition Dissociation Engineering and Technology Enzymes Genomics Hybridization Microscopy Nanoparticles Physical Sciences Research and analysis methods Ribonucleic acid RNA RNA sequencing Technology Tissues Transcriptomics United States
Online Access:	Get full text
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