Identification of a new gene, vanV , in vanB operons of Enterococcus faecalis

Abstract Subinhibitory concentrations of vancomycin are known to induce a cell-wall stimulon in some Gram-positive pathogens, but this has never been studied in the genus Enterococcus . In this study, Enterococcus faecalis V583 strain was submitted to a subinhibitory concentration of vancomycin. DNA...

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Published in:	International journal of antimicrobial agents Vol. 37; no. 6; pp. 554 - 557
Main Authors:	Ribeiro, Tânia, Santos, Sofia, Marques, Maria Isabel Martins, Gilmore, Michael, de Fátima Silva Lopes, Maria
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 01-06-2011
Subjects:	Anti-Bacterial Agents - pharmacology Enterococcus faecalis Enterococcus faecalis - drug effects Enterococcus faecalis - genetics Gene Expression Profiling Genes, Bacterial Infectious Disease Microarray Analysis microarray technology Operon pathogens transcription (genetics) Transcriptional Activation transcriptomics vanB operon vancomycin Vancomycin - pharmacology Vancomycin Resistance vanB operon Enterococcus faecalis Vancomycin resistance
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Abstract	Abstract Subinhibitory concentrations of vancomycin are known to induce a cell-wall stimulon in some Gram-positive pathogens, but this has never been studied in the genus Enterococcus . In this study, Enterococcus faecalis V583 strain was submitted to a subinhibitory concentration of vancomycin. DNA microarray technology was used to analyse the transcriptomic changes induced by this antibiotic. EF2292, annotated as a hypothetical protein in the E. faecalis V583 genome, was highly induced in response to vancomycin exposure, to similar levels as the vanB operon genes. We investigated further and provide evidence for co-transcription of ef2292 with vanY B WH B BX B genes. It was also demonstrated that expression of ef2292 is under the control of vanR B S B and it is proposed to name it vanV . This gene was found not to be required for vancomycin resistance under the conditions tested, thus coding for another accessory protein in the vanB operon. vanV was detected in some, but not all, E. faecalis carrying the vanB operon, suggesting that this operon can have different composition amongst E. faecalis isolates.
AbstractList	Subinhibitory concentrations of vancomycin are known to induce a cell-wall stimulon in some Gram-positive pathogens, but this has never been studied in the genus Enterococcus. In this study, Enterococcus faecalis V583 strain was submitted to a subinhibitory concentration of vancomycin. DNA microarray technology was used to analyse the transcriptomic changes induced by this antibiotic. EF2292, annotated as a hypothetical protein in the E. faecalis V583 genome, was highly induced in response to vancomycin exposure, to similar levels as the vanB operon genes. We investigated further and provide evidence for co-transcription of ef2292 with vanY(B)WH(B)BX(B) genes. It was also demonstrated that expression of ef2292 is under the control of vanR(B)S(B) and it is proposed to name it vanV. This gene was found not to be required for vancomycin resistance under the conditions tested, thus coding for another accessory protein in the vanB operon. vanV was detected in some, but not all, E. faecalis carrying the vanB operon, suggesting that this operon can have different composition amongst E. faecalis isolates. Subinhibitory concentrations of vancomycin are known to induce a cell-wall stimulon in some Gram-positive pathogens, but this has never been studied in the genus Enterococcus. In this study, Enterococcus faecalis V583 strain was submitted to a subinhibitory concentration of vancomycin. DNA microarray technology was used to analyse the transcriptomic changes induced by this antibiotic. EF2292, annotated as a hypothetical protein in the E. faecalis V583 genome, was highly induced in response to vancomycin exposure, to similar levels as the vanB operon genes. We investigated further and provide evidence for co-transcription of ef2292 with vanYBWHBBXB genes. It was also demonstrated that expression of ef2292 is under the control of vanRBSB and it is proposed to name it vanV. This gene was found not to be required for vancomycin resistance under the conditions tested, thus coding for another accessory protein in the vanB operon. vanV was detected in some, but not all, E. faecalis carrying the vanB operon, suggesting that this operon can have different composition amongst E. faecalis isolates. Subinhibitory concentrations of vancomycin are known to induce a cell-wall stimulon in some Gram-positive pathogens, but this has never been studied in the genus Enterococcus. In this study, Enterococcus faecalis V583 strain was submitted to a subinhibitory concentration of vancomycin. DNA microarray technology was used to analyse the transcriptomic changes induced by this antibiotic. EF2292, annotated as a hypothetical protein in the E. faecalis V583 genome, was highly induced in response to vancomycin exposure, to similar levels as the vanB operon genes. We investigated further and provide evidence for co-transcription of ef2292 with vanY B WH B BX B genes. It was also demonstrated that expression of ef2292 is under the control of vanR B S B and it is proposed to name it vanV. This gene was found not to be required for vancomycin resistance under the conditions tested, thus coding for another accessory protein in the vanB operon. vanV was detected in some, but not all, E. faecalis carrying the vanB operon, suggesting that this operon can have different composition amongst E. faecalis isolates. Abstract Subinhibitory concentrations of vancomycin are known to induce a cell-wall stimulon in some Gram-positive pathogens, but this has never been studied in the genus Enterococcus . In this study, Enterococcus faecalis V583 strain was submitted to a subinhibitory concentration of vancomycin. DNA microarray technology was used to analyse the transcriptomic changes induced by this antibiotic. EF2292, annotated as a hypothetical protein in the E. faecalis V583 genome, was highly induced in response to vancomycin exposure, to similar levels as the vanB operon genes. We investigated further and provide evidence for co-transcription of ef2292 with vanY B WH B BX B genes. It was also demonstrated that expression of ef2292 is under the control of vanR B S B and it is proposed to name it vanV . This gene was found not to be required for vancomycin resistance under the conditions tested, thus coding for another accessory protein in the vanB operon. vanV was detected in some, but not all, E. faecalis carrying the vanB operon, suggesting that this operon can have different composition amongst E. faecalis isolates.
Author	Santos, Sofia Gilmore, Michael de Fátima Silva Lopes, Maria Ribeiro, Tânia Marques, Maria Isabel Martins
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Cites_doi	10.1371/journal.pgen.0020096 10.1007/BF00262430 10.1007/s10096-009-0704-x 10.1128/AAC.44.3.705-709.2000 10.1016/0966-842X(96)10063-9 10.1371/journal.pone.0000582 10.1128/AAC.33.9.1588 10.1126/science.1080613 10.1128/JB.00773-06 10.1128/jb.176.1.260-264.1994 10.1128/JB.186.23.7951-7958.2004 10.1056/NEJM198807213190307 10.1128/JB.180.21.5792-5795.1998 10.1128/JB.00153-10
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Snippet	Abstract Subinhibitory concentrations of vancomycin are known to induce a cell-wall stimulon in some Gram-positive pathogens, but this has never been studied... Subinhibitory concentrations of vancomycin are known to induce a cell-wall stimulon in some Gram-positive pathogens, but this has never been studied in the...
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SubjectTerms	Anti-Bacterial Agents - pharmacology Enterococcus faecalis Enterococcus faecalis - drug effects Enterococcus faecalis - genetics Gene Expression Profiling Genes, Bacterial Infectious Disease Microarray Analysis microarray technology Operon pathogens transcription (genetics) Transcriptional Activation transcriptomics vanB operon vancomycin Vancomycin - pharmacology Vancomycin Resistance
Title	Identification of a new gene, vanV , in vanB operons of Enterococcus faecalis
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