Detection of a cytosolic glutamine synthetase in leaves of Nicotiana tabacum L. by immunocytochemical methods

• Published in: Plant physiology (Bethesda) Vol. 100; no. 3; pp. 1591 - 1594
• Main Authors: CARVALHO, H, PEREIRA, S, SUNKEL, C, SALEMA, R
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01-11-1992
• Subjects: Agronomy. Soil science and plant productions; Antibodies; Biological and medical sciences; Chloroplasts; citoplasma; Communications; cytoplasm; cytoplasme; feuille; Fundamental and applied biological sciences. Psychology; hojas; immunologie; immunology; inmunologia; Leaves; ligasas; ligase; ligases; Mesophyll cells; Metabolism; nicotiana tabacum; Nitrogen metabolism; Phloem; Phloem companion cells; Plant cells; Plant physiology and development; Plant tissues; Protein isoforms; Vascular tissues; Enzyme; Immunocytochemistry; Isozyme; Stimulant plant; Plant leaf; Glutamine synthetase; Nicotiana tabacum; Immunological method; Transmission electron microscopy; Dicotyledones; Angiospermae; Immunoblotting assay; Electrophoresis; Spermatophyta; Solanaceae; Localization; Chloroplast; Cytoplasm
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.100.3.1591

Two glutamine synthetase (GS) polypeptides (44 and 39 kD) were immunodetected on western blots of leaf extracts from tobacco (Nicotiana tabacum L.), a plant that has been reported to contain only chloroplast GS in the leaves. By immunocytochemical methods, we confirmed the localization of GS in the cytosol of cells in the vascular tissue and in the chloroplasts of mesophyll cells.