Antibacterial and antibiofilm activities of tryptoquivalines and meroditerpenes isolated from the marine-derived fungi Neosartorya paulistensis, N. laciniosa, N. tsunodae, and the soil fungi N. fischeri and N. siamensis

• Published in: Marine drugs Vol. 12; no. 2; pp. 822 - 839
• Main Authors: Gomes, Nelson M, Bessa, Lucinda J, Buttachon, Suradet, Costa, Paulo M, Buaruang, Jamrearn, Dethoup, Tida, Silva, Artur M S, Kijjoa, Anake
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28-01-2014; MDPI
• Subjects: Animals; Anti-Bacterial Agents - administration & dosage; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; antibacterial; antibiofilm; Bacillus subtilis; Bacteria - drug effects; Biofilms - drug effects; Diterpenes - administration & dosage; Diterpenes - isolation & purification; Diterpenes - pharmacology; Drug Resistance, Multiple, Bacterial; Drug Synergism; Escherichia coli; Indoles - administration & dosage; Indoles - isolation & purification; Indoles - pharmacology; Marine; marine-derived fungi; meroditerpenes; Microbial Sensitivity Tests; multidrug-resistant; Neosartorya; Neosartorya - chemistry; Porifera - microbiology; Pseudomonas aeruginosa; Soil Microbiology; Staphylococcus aureus; tryptoquivalines
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md12020822

A new meroditerpene, sartorypyrone C (5), was isolated, together with the known tryptoquivalines L (1a), H (1b), F (1c), 3'-(4-oxoquinazolin-3-yl) spiro [1H-indole-3,5']-2,2'-dione (2) and 4(3H)-quinazolinone (3), from the culture of the marine sponge-associated fungus Neosartorya paulistensis (KUFC 7897), while reexamination of the fractions remaining from a previous study of the culture of the diseased coral-derived fungus N. laciniosa (KUFC 7896) led to isolation of a new tryptoquivaline derivative tryptoquivaline T (1d). Compounds 1a-d, 2, 3, and 5, together with aszonapyrones A (4a) and B (4b), chevalones B (6) and C (7a), sartorypyrones B (7b) and A (8), were tested for their antibacterial activity against four reference strains (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa), as well as the environmental multidrug-resistant isolates. Only aszonapyrone A (4a) and sartorypyrone A (8) exhibited significant antibacterial activity as well as synergism with antibiotics against the Gram-positive multidrug-resistant strains. Antibiofilm assays of aszonapyrone A (4a) and sartorypyrone A (8) showed that practically no biofilm was formed in the presence of their 2× MIC and MIC. However, the presence of a sub-inhibitory concentration of ½ MIC of 4a and 8 was found to increase the biofilm production in both reference strain and the multidrug-resistant isolates of S. aureus.