Shiga toxin‐producing Escherichia coli and enteropathogenic Escherichia coli in healthy goats in India: occurrence and virulence properties

• Published in: Journal of applied microbiology Vol. 100; no. 1; pp. 108 - 113
• Main Authors: Wani, S.A., Samanta, I., Munshi, Z.H., Bhat, M.A., Nishikawa, Y.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01-01-2006; Blackwell Science; Oxford University Press
• Subjects: Animals; Animals, Wild - microbiology; Biological and medical sciences; Disease Reservoirs; enteropathogenic Escherichia coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli - metabolism; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Genes, Bacterial - genetics; goats; Goats - microbiology; healthy; India; Microbiology; multiplex‐PCR; Serotyping; Shiga Toxins - biosynthesis; shiga toxin‐producing Escherichia coli; Virulence - genetics; Asia; India; Applied microbiology; Escherichia coli; Virulence; goats; multiplex-PCR; Polymerase chain reaction; shiga toxin-producing Escherichia coli; Vertebrata; enteropathogenic Escherichia coli; Mammalia; healthy; Bacteria; Goat; Artiodactyla; Ungulata; Shiga like toxin; Enterobacteriaceae
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2005.02759.x

Aims:  To describe the occurrence and virulence gene pattern of shiga toxin‐producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) in healthy goats of Jammu and Kashmir, India.
Methods and Results:  A total of 220 E. coli strains belonging to 60 different ‘O’ serogroups was isolated from 206 local (nonmigratory) and 69 migratory goats. All the 220 strains were screened for the presence of stx1, stx2, eaeA and hlyA genes. Twenty‐eight E. coli (75·6%) strains from local and nine (24·3%) strains from migratory goats belonging to 18 different serogroups showed at least presence of one virulence gene studied. Twenty‐eight strains (16·47%) (belonging to 13 different serogroups) from local goats carried stx1 gene alone or in combination with stx2 gene, while as only one strain (2%) from migratory goats possessed stx2 gene alone. Interestingly in the present study none of the STEC strains carried eaeA gene. Similarly, none of the strains from local goats possessed eaeA and none of the migratory goats possessed stx1 gene. Eight strains (16%) (belonging to four different serogroups) from migratory goats carried eaeA gene. Twenty‐five (14·7%) and seven (14%) strains from local and migratory goats harboured hlyA gene respectively.
Conclusions:  Healthy goats of Jammu and Kashmir state serve as a reservoir of STEC and EPEC. Further studies in this direction are needed to work out whether or not they are transmitted to humans in this part of world.
Significance and Impact of the Study:  This study is the first report of isolation of STEC and EPEC strains from healthy goats in Jammu and Kashmir State of India, which could be a source of infection to humans.