Isolation of a ripening and wound-induced cDNA from Cucumis melo L. encoding a protein with homology to the ethylene-forming enzyme

A cDNA clone (pMEL1) was isolated from a climacteric melon fruit cDNA library using the tomato ethylene-forming-enzyme (EFE) cDNA, pTOM13, as a probe. Northern analysis of RNA isolated from wounded leaf and fruit tissue and from preclimacteric and climacteric pericarp tissue was used to determine th...

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Bibliographic Details
Published in:	European journal of biochemistry Vol. 212; no. 1; pp. 27 - 34
Main Authors:	Balague, C, Watson, C.F, Turner, A.J, Rouge, P, Picton, S, Pech, J.C, Grierson, D
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 15-02-1993 Blackwell
Subjects:	Amino Acid Sequence amino acid sequences Base Sequence Biological and medical sciences Blotting, Northern Blotting, Southern Blotting, Western climacteric fruit clones complementary DNA Cucumis melo DNA libraries DNA Probes enzymes ethylene production Ethylenes - biosynthesis Fructification and ripening Fruit - genetics Fruit - metabolism Fundamental and applied biological sciences. Psychology genbank/x69935 genetic code injuries leaves Lyases - genetics melons Molecular Sequence Data nucleotide sequences pericarp Plant physiology and development plant proteins Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - immunology ripening Sequence Homology, Amino Acid shelf life Vegetative and sexual reproduction, floral biology, fructification Fruit Enzyme Gene product Injury Cucurbitaceae Ethylene Homology Primary structure Biosynthesis Gene expression Ripening Tissue Complementary DNA Dicotyledones Angiospermae Plant growth substance Spermatophyta Cucumis melo
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