Proteome profiling of peripheral mononuclear cells from human blood

• Published in: Proteomics (Weinheim) Vol. 13; no. 5; pp. 893 - 897
• Main Authors: Maccarrone, Giuseppina, Rewerts, Christiane, Lebar, Maria, Turck, Christoph W., Martins-de-Souza, Daniel
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01-03-2013; Wiley Subscription Services, Inc
• Subjects: Biomarkers; Biomedicine; Blood; Blood Proteins - analysis; Blood Proteins - chemistry; Databases, Protein; Diseases; Electrophoresis, Polyacrylamide Gel; Free radicals; Humans; Leukocytes, Mononuclear - chemistry; Medical research; Mononuclear cells; Peptide Mapping; Proteins; Proteome - analysis; Proteomics; Shotgun
• ISSN: 1615-9853; 1615-9861
• DOI: 10.1002/pmic.201200377

Peripheral blood mononuclear cells (MNCs) are accessible through blood collection and represent a useful source for investigations on disease mechanisms and treatment response. Aiming to build a reference proteome database, we generated three proteome data sets from MNCs using a combination of SDS‐PAGE and nanoflow LC‐MS. Experiments were performed in triplicates and 514 unique proteins were identified by at least two non‐redundant peptides with 95% confidence for all replicates. Identified proteins are associated with a range of dermatologic, inflammatory and neurological conditions as well as molecular processes, such as free radical scavenging and cellular growth and proliferation. Mapping the MNC proteome provides a valuable resource for studies on disease pathogenesis and the identification of therapeutic targets.