The Utility of Serum Hepcidin as a Biomarker for Late-Onset Neonatal Sepsis

• Published in: The Journal of pediatrics Vol. 162; no. 1; pp. 67 - 71
• Main Authors: Wu, Tai-Wei, MD, Tabangin, Meredith, MPH, Kusano, Ryosuke, MD, Ma, Yan, BS, Ridsdale, Ross, PhD, Akinbi, Henry, MD
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 2013; Mosby, Inc
• Subjects: Age of Onset; antibiotics; Antimicrobial Cationic Peptides - blood; biomarkers; Biomarkers - blood; blood serum; C-reactive protein; C-Reactive Protein - analysis; Female; hepcidin; Hepcidins; Humans; Infant, Newborn; Infant, Very Low Birth Weight; infants; inflammation; low birth weight; Male; Pediatrics; Predictive Value of Tests; regression analysis; Sepsis - blood; Sepsis - diagnosis; therapeutics; CRP; ln; C-reactive protein; VLBW; Very low birth weight; Negative predictive value; Classification and regression tree; Natural logarithmic; Coefficient of variation; CV; NPV; PPV; Positive predictive value; CART
• ISSN: 0022-3476; 1097-6833
• DOI: 10.1016/j.jpeds.2012.06.010

Objective To assess the utility of hepcidin, a potent regulator of host defense and inflammation, in the diagnosis of late-onset sepsis in very low birth weight infants. Study design We compared the diagnostic performance of hepcidin with C-reactive protein from the serum concentrations in acute and convalescent blood specimens obtained from 44 infants suspected of late-onset sepsis. The predictive accuracies were assessed from the areas under receiver operating characteristic curves and the cutoffs that differentiated infants with and without sepsis were identified using classification and regression tree analysis. Results Seventeen of the enrolled infants in this study were bacteremic and/or received antibiotics for neonatal sepsis for ≥5 days (infants with sepsis). The concentrations of hepcidin were increased 4-fold in infants with compared with infants without sepsis ( P  < .0001) and returned to similar levels following therapy. The areas under receiver operating characteristic curves of hepcidin was 0.93 compared with 0.83 for C-reactive protein, P  = .06. Hepcidin concentration >92.2 ng/mL correctly classified 91% of all infants (positive predictive value: 100%, negative predictive value: 87%, specificity: 100%, and sensitivity: 76%). Conclusion Serum hepcidin concentration may be a useful adjunct test, in addition to blood culture and other markers of infection, in the evaluation of late-onset sepsis in very low birth weight infants.