At least two Fc Neu5Gc residues of monoclonal antibodies are required for binding to anti-Neu5Gc antibody

Two non-human glycan epitopes, galactose-α-1,3-galactose (α-gal) and Neu5Gc-α-2-6-galactose (Neu5Gc) have been shown to be antigenic when attached to Fab oligosaccharides of monoclonal antibodies (mAbs) , while α-gal attached to Fc glycans was not. However, the antigenicity of Neu5Gc on the Fc glyca...

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Bibliographic Details
Published in:	Scientific reports Vol. 6; no. 1; p. 20029
Main Authors:	Yu, Chuanfei, Gao, Kai, Zhu, Lei, Wang, Wenbo, Wang, Lan, Zhang, Feng, Liu, Chunyu, Li, Meng, Wormald, Mark R., Rudd, Pauline M., Wang, Junzhi
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 29-01-2016 Nature Publishing Group
Subjects:	631/154/1438 631/61/51/1568 82/16 Acids Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antigenicity Biological products Carbohydrate Sequence Epitopes Epitopes - immunology Fab Galactose Galactose - immunology Humanities and Social Sciences Humans Immunoglobulin Fc Fragments - immunology Immunoglobulin G - immunology Mice Monoclonal antibodies multidisciplinary Multiple myeloma Myeloma Oligosaccharides Oligosaccharides - immunology Polysaccharides Polysaccharides - immunology Quality control Science
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Summary:	Two non-human glycan epitopes, galactose-α-1,3-galactose (α-gal) and Neu5Gc-α-2-6-galactose (Neu5Gc) have been shown to be antigenic when attached to Fab oligosaccharides of monoclonal antibodies (mAbs) , while α-gal attached to Fc glycans was not. However, the antigenicity of Neu5Gc on the Fc glycans remains unclear in the context that most mAbs carry only Fc glycans. After studying two clinical mAbs carrying significant amounts of Fc Neu5Gc, we show that their binding activity with anti-Neu5Gc antibody resided in a small subset of mAbs carrying two or more Fc Neu5Gc, while mAbs harboring only one Neu5Gc showed no reactivity. Since most Neu5Gc epitopes were distributed singly on the Fc of mAbs, our results suggest that the potential antigenicity of Fc Neu5Gc is low. Our study could be referenced in the process design and optimization of mAb production in murine myeloma cells and in the quality control of mAbs for industries and regulatory authorities.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work.
ISSN:	2045-2322 2045-2322
DOI:	10.1038/srep20029