Biological detoxification of waste house wood hydrolysate using Ureibacillus thermosphaericus for bioethanol production

• Published in: Journal of bioscience and bioengineering Vol. 106; no. 2; pp. 128 - 133
• Main Authors: Okuda, Naoyuki, Soneura, Mayumi, Ninomiya, Kazuaki, Katakura, Yoshio, Shioya, Suteaki
• Format: Journal Article
• Language: English
• Published: Amsterdarm Elsevier B.V 01-08-2008; Elsevier Science; Elsevier Limited
• Subjects: ALCOHOLIC FERMENTATION; Bacillus - metabolism; bioethanol; Biological and medical sciences; Biotechnology; CALCIUM HYDROXIDE; Culture Media; DECHET DE BOIS; DESECHOS DE LA MADERA; DESINTOXICACION; DETOXIFICATION; Escherichia coli KO11; ETANOL; ETHANOL; Ethanol - metabolism; FERMENTACION ALCOHOLICA; Fermentation; FERMENTATION ALCOOLIQUE; Fundamental and applied biological sciences. Psychology; HIDROXIDO DE CALCIO; Hydrolysis; HYDROXYDE DE CALCIUM; LIGNOCELLULOSE; LIGNOCELULOSA; MICRO-ORGANISME THERMOPHILE; MICROORGANISMOS TERMOFILOS; SACCHAROMYCES CEREVISIAE; THERMOPHILIC MICROORGANISMS; Ureibacillus thermosphaericus; waste house wood; Wood - microbiology; WOOD RESIDUES; waste house wood; Ureibacillus thermosphaericus; bioethanol; detoxification; Saccharomyces cerevisiae; Escherichia coli KO11; Ascomycota; Yeast; Wood; Ethanol; Escherichia coli; Detoxification; Bioethanol; Fungi; Hydrolysate; Production; Bacteria; Wastes; Enterobacteriaceae
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1263/jbb.106.128

Hydrolysates of lignocelluloses hydrolyzed by diluted sulfuric acid contain toxic compounds that inhibit ethanol production by
Saccharomyces cerevisiae and the ethanologenic recombinant
Escherichia coli KO11. We investigated the biological detoxification of a hydrolysate of waste house wood (WHW) by a thermophilic bacterium,
Ureibacillus thermosphaericus. When the hydrolysate was treated with this bacterium at 50°C for 24 h, the ethanol production rate by
S. cerevisiae increased markedly and was comparable to that for the hydrolysate treated with an excess amount of calcium hydroxide (overliming). Chromatographic analysis of synthetic hydrolysates containing furfural or 5-hydroxymethyl furfural that are considered to be major toxic compounds in hydrolysates revealed that
U. thermosphaericus degrades these compounds. In the WHW hydrolysates, however, the concentrations of these compounds were not decreased markedly by the bacterium. These results suggest that the bacterium degrades minor but more toxic compounds or phenolic compounds in the WHW hydrolysates. The combination of bacterial and overliming treatments of hydrolysates minimized significantly the decrease in ethanol production rate by
E. coli KO11 as fermentation proceeded. Because the bacterium grows rapidly and does not consume sugars, our biological detoxification should be useful for bioethanol production from acid hydrolysates of lignocelluloses.