Generation of baculovirus vectors for the high-throughput production of proteins in insect cells

The baculovirus expression system is one of the most popular methods used for the production of recombinant proteins but has several complex steps which have proved inherently difficult to adapt to a multi-parallel process. We have developed a bacmid vector that does not require any form of selectio...

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Bibliographic Details
Published in:	Biotechnology and bioengineering Vol. 101; no. 6; pp. 1115 - 1122
Main Authors:	Possee, Robert D, Hitchman, Richard B, Richards, Kevin S, Mann, Susan G, Siaterli, Evangelia, Nixon, Clare P, Irving, Helen, Assenberg, Rene, Alderton, David, Owens, Raymond J, King, Linda A
Format:	Journal Article
Language:	English
Published:	Hoboken Wiley Subscription Services, Inc., A Wiley Company 15-12-2008 Wiley Wiley Subscription Services, Inc
Subjects:	Animals automated bacmid Baculoviridae - genetics Baculovirus baculovirus expression system Biochemistry Biological and medical sciences Biotechnology Biotechnology - methods Cell Line Cells Cloning, Molecular Fundamental and applied biological sciences. Psychology Gene Expression Genes Genetic Vectors high throughput Insecta Insects Molecular Biology - methods Proteins Recombinant Proteins - genetics Recombination, Genetic robotic liquid handler secreted protein Virology Virus Replication Viruses Automation bacmid Insecta baculovirus expression system Gene expression high throughput Robotics secreted protein Virus automated Baculoviridae Arthropoda Production Secretory protein robotic liquid handler Invertebrata Vector
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