Effect of Bradykinin Postconditioning on Ischemic and Toxic Brain Damage

• Published in: Neurochemical research Vol. 40; no. 8; pp. 1728 - 1738
• Main Authors: Lalkovičová, Mária, Bonová, Petra, Burda, Jozef, Danielisová, Viera
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-08-2015; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bradykinin - pharmacology; Bradykinin - therapeutic use; Brain Ischemia - drug therapy; Brain Ischemia - metabolism; Cell Biology; Hypoxia, Brain - drug therapy; Hypoxia, Brain - metabolism; Ischemic Postconditioning - methods; Male; Maze Learning - drug effects; Maze Learning - physiology; Neurochemistry; Neurology; Neuroprotective Agents - pharmacology; Neuroprotective Agents - therapeutic use; Neurosciences; Original Paper; Rats; Rats, Wistar; Post-conditioning; Intoxication; Ischemia; Glutamate; Bradykinin
• ISSN: 0364-3190; 1573-6903
• DOI: 10.1007/s11064-015-1675-1

Brain damage caused by ischemia or toxic agents leads in selectively vulnerable regions to apoptosis-like delayed neuronal death and can result in irreversible damage. Selectively vulnerable neurons of the CA1 area of hippocampus are particularly sensitive to ischemic damage. We investigated the effects of bradykinin (BR) postconditioning on cerebral ischemic and toxic injury. Transient forebrain ischemia was induced by four-vessel occlusion for 10 min and toxic injury was induced by trimethyltin (TMT, 8 µg/kg i.p.). BR as a postconditioner at a dose of 150 µg/kg was applied intraperitoneally 48 h after ischemia or TMT intoxication. Experimental animals were divided into groups according to the length of survival (short—3 and 7 days, and long—28 days survival) and according to the applied ischemic or toxic injury. Glutamate concentration was lowered in both CA1 and dentate gyrus areas of hippocampus after the application of BR postconditioning in both ischemic and toxic brain damage. The number of degenerated neurons in the hippocampal CA1 region was significantly lower in BR-treated ischemic and toxic groups compared to vehicle group. The behavioral test used in our experiments confirms also the memory improvement in conditioned animals. The rats’ ability to form spatial maps and learn was preserved, which is visible from our Barnes maze results. By using the methods of delayed postconditioning is possible to stimulate the endogenous protective mechanisms of the organism and induce the neuroprotective effect. In this study we demonstrated that BR postconditioning, if applied before the onset of irreversible neurodegenerative changes, induced neuroprotection against ischemic or toxic injury.