The combination of mesenchymal stem cells and a bone scaffold in the treatment of vertebral body defects

• Published in: European spine journal Vol. 22; no. 12; pp. 2777 - 2786
• Main Authors: Vaněček, Václav, Klíma, Karel, Kohout, Aleš, Foltán, René, Jiroušek, Ondřej, Šedý, Jiří, Štulík, Jan, Syková, Eva, Jendelová, Pavla
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-12-2013; Springer Nature B.V
• Subjects: Anesthesia; Animals; Bone Transplantation - methods; Disease Models, Animal; Durapatite; Humans; Lumbar Vertebrae - diagnostic imaging; Lumbar Vertebrae - injuries; Lumbar Vertebrae - pathology; Male; Medicine; Medicine & Public Health; Mesenchymal Stem Cell Transplantation - methods; Neurosurgery; Original; Original Article; Radiography; Random Allocation; Rats; Rats, Wistar; Spinal Injuries - diagnostic imaging; Spinal Injuries - pathology; Spinal Injuries - therapy; Surgical Orthopedics; Tissue Engineering - methods; Tissue Scaffolds; Wound Healing; Rat model; Vertebral body defect; Hydroxyapatite scaffold; Mesenchymal stem cells
• DOI: 10.1007/s00586-013-2991-2
• EISSN: 1432-0932
• ISSN: 0940-6719

Purpose
Vertebral body defects represent one of the most common orthopedic challenges. In order to advance the transfer of stem cell therapies into orthopedic clinical practice, we performed this study to evaluate the safety and efficacy of a composite bioartificial graft based on a hydroxyapatite bone scaffold (CEM-OSTETIC
®
) combined with human mesenchymal stem cells (MSCs) in a rat model of vertebral body defects.
Methods
Under general isoflurane anesthesia, a defect in the body of the L
2
vertebra was prepared and left to heal spontaneously (group 1), implanted with scaffold material alone (group 2), or implanted with a scaffold together with 0.5 million MSCs (group 3) or 5 million MSCs (group 4). The rats were killed 8 weeks after surgery. Histological and histomorphometrical evaluation of the implant as well as micro-CT imaging of the vertebrae were performed.
Results
We observed a significant effect on the formation of new bone tissue in the defect in group 4 when compared to the other groups and a reduced inflammatory reaction in both groups receiving a scaffold and MSCs. We did not detect any substantial pathological changes or tumor formation after graft implantation.
Conclusions
MSCs in combination with a hydroxyapatite scaffold improved the repair of a model bone defect and might represent a safe and effective alternative in the treatment of vertebral bone defects.