Identification of two mosquitocidal Bacillus cereus strains showing different host ranges

• Published in: Journal of invertebrate pathology Vol. 100; no. 1; pp. 54 - 56
• Main Authors: Park, Hyun-Woo, Hayes, Sabrina R., Stout, Gail M., Day-Hall, Ginny, Latham, Mark D., Hunter, John P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 2009; Elsevier
• Subjects: animal parasites and pests; Animals; Bacillus cereus; Bacillus cereus - chemistry; Bacillus cereus - isolation & purification; Bacillus cereus - physiology; Base Sequence; Biological and medical sciences; biological control; biological control agents; Chromatography, Gas; Culex quinquefasciatus; Culicidae; Culicidae - drug effects; Culicidae - growth & development; Electrophoresis, Polyacrylamide Gel; Endotoxins - chemistry; Endotoxins - toxicity; entomopathogenic bacteria; Fundamental and applied biological sciences. Psychology; Geologic Sediments - microbiology; host range; hosts; insect pests; instars; Larva - drug effects; Larva - growth & development; larvae; Lethal Dose 50; Medically important nuisances and vectors, pests of stored products and materials: population survey and control; molecular sequence data; Mosquitocidal; nucleotide sequences; Ochlerotatus; Ochlerotatus taeniorhynchus; Pest Control, Biological; ribosomal RNA; RNA, Bacterial - chemistry; RNA, Ribosomal - chemistry; Sequence Alignment; Sequence Analysis, RNA; species differences; sporulation; strain differences; strains; Vectors. Intermediate hosts; Mosquitocidal; Ochlerotatus taeniorhynchus; Bacillus cereus; Culex quinquefasciatus; Entomopathogen; Disease; Insecta; Parasite; Bacillaceae; Bacillales; Arthropoda; Culicidae; Host range; Bacteria; Ectoparasite; Invertebrata; Vector; Diptera
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/j.jip.2008.09.010

Mosquitocidal bacteria, M413 and C32 have been isolated from sediment samples collected from woodland and ditch, respectively. Gas chromatographic analysis of fatty acids methyl esters (GC-FAME) and 16S rRNA gene sequence alignment results showed these isolates belong to
Bacillus cereus. The SDS–PAGE analysis of sporulated cultures of both isolates showed two major bands very similar in size. Interestingly, however, M413 is mainly toxic to 4th instars of
Ochlerotatus taeniorhynchus whereas C32 is to those of
Culex quinquefasciatus.