Defensive Effects of Human Antimicrobial Peptide α-Defensins against Enterococcus faecalis
Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faec...
Saved in:
| Published in: | Journal of Health Science Vol. 56; no. 5; pp. 618 - 622 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
The Pharmaceutical Society of Japan
2010
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans. |
|---|---|
| AbstractList | Cationic and amphiphilic antimicrobial peptides (AMPs) such as alpha -defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and alpha -defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The alpha -defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither ordefensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the alpha -defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that alpha -defensins may be defensive factors against E. faecalis in humans. Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we examined the protective effects of two AMPs, human neutrophil peptide-3 and α-defensin-5, against the opportunistic pathogen Enterococcus faecalis (E. faecalis). The α-defensins had dose-dependent bactericidal activity, whereas they showed no synergistic effect on the antimicrobial actions of antibiotics. Although AMPs often neutralize bacterial bioactive products, neither α-defensin reduced the proteolytic activity of GelE, a toxic protease from E. faecalis. On the other hand, the α-defensins were found to be fairly stable even in the presence of excess amounts of GelE. These results indicate that α-defensins may be defensive factors against E. faecalis in humans. |
| Author | Miyoshi, Shin-ichi Kashihara, Minoru Mizuno, Tamaki Maehara, Yoko Koyama, Kenta Nakao, Hiroshi |
| Author_xml | – sequence: 1 fullname: Miyoshi, Shin-ichi organization: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University – sequence: 2 fullname: Koyama, Kenta organization: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University – sequence: 3 fullname: Mizuno, Tamaki organization: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University – sequence: 4 fullname: Kashihara, Minoru organization: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University – sequence: 5 fullname: Maehara, Yoko organization: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University – sequence: 6 fullname: Nakao, Hiroshi organization: Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University |
| BookMark | eNo9kM9KAzEQxoNU8O_FJ8hNELZms5tscpKi1QqCHvTkIUyns23KNls3qeBj-SI-k6utXuYbmN838H1HbBDaQIyd5WKYy9JcLhdxqPRQ52aPHeZFWWVKimrwu5eZrYQ8YEcxLoWQVpj8kL3eUE0h-nfi47omTJG3NZ9sVhD4KCS_8ti1Uw8Nf6J18jPiX5_ZzhMihzn0kvg4JOpabBE3kddACI2PJ2y_hibS6U6P2cvt-Pl6kj083t1fjx4yVEKmzEqlZtqiICtLQJxpgShgikVRTkGjFNLkhUFUupppUNpYo3JpC1MXYKUpjtn59u-6a982FJNb-YjUNBCo3URntFWV0KLsyYst2YeKsaParTu_gu7D5cL9FOj6Ap3Sri-wh6-28DImmNM_Cl3y2NAfqrajd_xfcAGdo1B8A9Z9fWA |
| CitedBy_id | crossref_primary_10_4265_bio_19_199 |
| Cites_doi | 10.1128/IAI.00232-09 10.4265/bio.9.77 10.1111/j.0019-2805.2004.01837.x 10.1128/CMR.7.4.462 10.1146/annurev.immunol.13.1.61 10.1128/IAI.59.1.415-420.1991 10.1128/AAC.48.12.4673-4679.2004 10.1128/AAC.01488-05 10.1016/j.smim.2007.04.002 10.3109/15569549709016455 10.1073/pnas.0500508102 10.1128/CMR.13.4.686-707.2000 10.1111/j.1348-0421.1987.tb03064.x 10.1038/415389a 10.1128/CMR.13.4.513-522.2000 10.1016/j.ijfoodmicro.2006.09.004 10.1007/s00018-005-5034-2 10.1128/jb.179.23.7606-7609.1997 10.1002/bip.20543 10.1128/CMR.3.1.46 10.1016/S0021-9258(18)60449-1 10.1159/000007174 10.1038/nbt1267 10.1002/psc.704 |
| ContentType | Journal Article |
| Copyright | 2010 by The Pharmaceutical Society of Japan |
| Copyright_xml | – notice: 2010 by The Pharmaceutical Society of Japan |
| DBID | AAYXX CITATION 7QL 7T7 8FD C1K FR3 P64 |
| DOI | 10.1248/jhs.56.618 |
| DatabaseName | CrossRef Bacteriology Abstracts (Microbiology B) Industrial and Applied Microbiology Abstracts (Microbiology A) Technology Research Database Environmental Sciences and Pollution Management Engineering Research Database Biotechnology and BioEngineering Abstracts |
| DatabaseTitle | CrossRef Engineering Research Database Technology Research Database Industrial and Applied Microbiology Abstracts (Microbiology A) Bacteriology Abstracts (Microbiology B) Biotechnology and BioEngineering Abstracts Environmental Sciences and Pollution Management |
| DatabaseTitleList | Engineering Research Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Public Health |
| EISSN | 1347-5207 |
| EndPage | 622 |
| ExternalDocumentID | 10_1248_jhs_56_618 article_jhs_56_5_56_5_618_article_char_en |
| GroupedDBID | --- .55 29K 2WC 53G 5GY AAUGY ADBBV AFRAH ALMA_UNASSIGNED_HOLDINGS BAWUL BKOMP CS3 D-I DIK DU5 E3Z EBS EJD FRP JMI JSF JSH KQ8 MOJWN M~E OK1 P2P RJT RYR RZJ TKC TR2 W2D X7M XSB AAYXX CITATION 7QL 7T7 8FD C1K FR3 P64 |
| ID | FETCH-LOGICAL-c502t-9255d69c0e924accd60cc0abc334ba6c2028138cc567d6a56898512938f3a9283 |
| ISSN | 1344-9702 |
| IngestDate | Fri Jun 28 17:35:06 EDT 2024 Fri Aug 23 01:25:11 EDT 2024 Thu Aug 17 20:29:34 EDT 2023 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 5 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c502t-9255d69c0e924accd60cc0abc334ba6c2028138cc567d6a56898512938f3a9283 |
| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
| OpenAccessLink | https://www.jstage.jst.go.jp/article/jhs/56/5/56_5_618/_article/-char/en |
| PQID | 869570604 |
| PQPubID | 23462 |
| PageCount | 5 |
| ParticipantIDs | proquest_miscellaneous_869570604 crossref_primary_10_1248_jhs_56_618 jstage_primary_article_jhs_56_5_56_5_618_article_char_en |
| PublicationCentury | 2000 |
| PublicationDate | 2010-00-00 |
| PublicationDateYYYYMMDD | 2010-01-01 |
| PublicationDate_xml | – year: 2010 text: 2010-00-00 |
| PublicationDecade | 2010 |
| PublicationTitle | Journal of Health Science |
| PublicationYear | 2010 |
| Publisher | The Pharmaceutical Society of Japan |
| Publisher_xml | – name: The Pharmaceutical Society of Japan |
| References | 2) Zasloff, M. (2002) Antimicrobial peptides of multicellular organisms. Nature, 415, 389-395. 11) Thwaite, J. E., Hibbs, S., Titball, R. W. and Atkins, T. P. (2006) Proteolytic degradation of human antimicrobial peptide LL-37 by Bacillus anthracis may contribute to virulence. Antimicrob. Agents Chemother., 50, 2316-2322. 22) Wilde, C. G., Griffith, J. E., Marra, M. N., Snable, J. L. and Scott, R. W. (1989) Purification and characterization of human neutrophil peptide 4, a novel member of the defensin family. J. Biol. Chem., 264, 11200-11203. 15) Jett, B. D., Huycke, M. M. and Gilmore, M. S. (1994) Virulence of enterococci. Clin. Microbiol. Rev., 7, 462-478. 7) Chun, K., Nadesan, G., Hans-Willi, M., Matthias, W., Young-Hwa, S., Robert, H., Matthias, W., Gunter, F. and Stefan, H. E. K. (2005) Human α-defensins neutralize anthrax lethal toxin and protect against its fatal consequences. Proc. Natl. Acad. Sci. U.S.A., 102, 4830-4835. 4) Hancock, R. E. W. and Sahl, H. G. (2006) Antimicrobial and host-defense peptides as new antiinfective therapeutic strategies. Nature Biotech., 24, 1551-1557. 21) Fernie-King, B. A., Seilly, D. J. and Lachmann, P. J. (2004) The interaction of streptococcal inhibitor of complement (SIC) and its proteolytic fragments with the human beta defensins. Immunology, 111, 444-452. 1) Boman, H. G. (1995) Peptide antibiotics and their role in innate immunity. Annu. Rev. Immunol., 13, 61-92. 23) Miyoshi, S., Wakae, H., Tomochika, K. and Shinoda, S. (1997) Functional domains of a zinc metalloprotease from Vibrio vulnificus. J. Bacteriol., 179, 7606-7609. 24) Miyoshi, S. and Shinoda, S. (1997) Bacterial metalloprotease as the toxic factor in infection. J. Toxicol. Toxin Rev., 16, 174-199. 6) Mcphee, J. B. and Hancock, R. E. W. (2005) Function and therapeutic potential of host defence peptides. J. Pept. Sci., 11, 677-687. 13) Murray, B. E. (1990) The life and times of the Enterococcus. Clin. Microbiol. Rev., 3, 46-65. 8) Lehrer, R. I., Jung, G., Ruchala, P., Wang, W., Micewicz, E. D., Waring, A. J., Gillespie, E. J., Bradley, K. A., Ratner, A. J., Rest, R. F. and Lu, W. (2009) Human α-defensins inhibit hemolysis mediated by cholesterol-dependent cytolysins. Infect. Immun., 77, 4028-4040. 9) Yount, N. Y., Bayer, A. S., Xiong, Y. Q. and Yeaman, M. R. (2006) Advances in antimicrobial peptide immunobiology. Biopolymers, 84, 435-458. 10) Sieprawska-Lupa, M., Mydel, P., Krawezyk, K., Wojcik, K., Puklo, M., Lupa, B., Sunder, P., Silberring, J., Reed, M., Pohl, J., Shafer, W., McAleese, F., Foster, T., Travis, J. and Potempa, J. (2004) Degradation of human antimicrobial peptide LL-37 by Staphylococcus aureus-deriveed proteinases. Antimicrob. Agents Chemother., 48, 4673-4679. 12) Witte, W., Wirth, R. and Klare, I. (1999) Enterococci. Chemotherapy, 45, 135-145. 18) De Maria, F. S. L., Ana, P. S., Rogério, T., Jose, J. F. M. and Maria, T. B. C. (2006) Activity and expression of a virulence factor, gelatinase, in dairy enterococci. Int. J. Food Microbiol., 112, 208-214. 20) Miyoshi, N., Shimizu, C., Miyoshi, S. and Shinoda, S. (1987) Purification and characterization of Vibrio vulnificus protease. Microbiol. Immunol., 31, 13-25. 3) Muller, C. A., Autenrieth, I. B. and Peschel, A. (2005) Innate defenses of the intestinal epithelial barrier. Cell. Mol. Life Sci., 62, 1297-1307. 19) Su, Y. A., Sulavik, M. C., He, P., Makinen, K. K., Makinen, P. L., Fiedler, S., Wirth, R. and Clewell, D. B. (1991) Nucleotide sequence of the gelatinase gene (gelE) from Enterococcus faecalis subsp. liquefaciens. Infect. Immun., 59, 415-420. 5) Salzman, N. H., Underwood, M. A. and Bevins, C. L. (2007) Paneth cells, defensins, and the commensal microbiota: a hypothesis on intimate interplay as the intestinal mucosa. Semin. Immunol., 19, 70-83. 14) Ohhira, I., Kuwaki, S., Morita, H., Suzuki, T., Tomita, S., Hisamatsu, S., Sonoki, S. and Shinoda, S. (2004) Identification of 3-phenyllactic acid as a possible antimicrobial substance produced by Enterococcus faecalis TH10. Biocontrol Sci., 9, 77-81. 17) Cetinkaya, Y., Falk, P. and Mayhall, C. G. (2000) Vancomycin-resistant enterococci. Clin. Microbiol. Rev., 13, 686-707. 16) Mundy, L. M., Sahm, D. F. and Gilmore, M. (2000) Relationship between enterococcal virulence and antimicrobial resistance. Clin. Microbiol. Rev., 13, 513-522. 11 12 13 24 (15) 1994; 7 16 17 18 19 (22) 1989; 264 MIYOSHI N (20) 1987; 31 1 2 (23) 1997; 179 3 4 5 6 7 8 9 OHHIRA I (14) 2004; 9 10 21 |
| References_xml | – ident: 8 doi: 10.1128/IAI.00232-09 – volume: 9 start-page: 77 issn: 1342-4815 issue: 3 year: 2004 ident: 14 doi: 10.4265/bio.9.77 contributor: fullname: OHHIRA I – ident: 21 doi: 10.1111/j.0019-2805.2004.01837.x – volume: 7 start-page: 462 issn: 0893-8512 issue: 4 year: 1994 ident: 15 doi: 10.1128/CMR.7.4.462 – ident: 1 doi: 10.1146/annurev.immunol.13.1.61 – ident: 19 doi: 10.1128/IAI.59.1.415-420.1991 – ident: 10 doi: 10.1128/AAC.48.12.4673-4679.2004 – ident: 11 doi: 10.1128/AAC.01488-05 – ident: 5 doi: 10.1016/j.smim.2007.04.002 – ident: 24 doi: 10.3109/15569549709016455 – ident: 7 doi: 10.1073/pnas.0500508102 – ident: 17 doi: 10.1128/CMR.13.4.686-707.2000 – volume: 31 start-page: 13 issn: 0385-5600 issue: 1 year: 1987 ident: 20 doi: 10.1111/j.1348-0421.1987.tb03064.x contributor: fullname: MIYOSHI N – ident: 2 doi: 10.1038/415389a – ident: 16 doi: 10.1128/CMR.13.4.513-522.2000 – ident: 18 doi: 10.1016/j.ijfoodmicro.2006.09.004 – ident: 3 doi: 10.1007/s00018-005-5034-2 – volume: 179 start-page: 7606 issn: 0021-9193 issue: 23 year: 1997 ident: 23 doi: 10.1128/jb.179.23.7606-7609.1997 – ident: 9 doi: 10.1002/bip.20543 – ident: 13 doi: 10.1128/CMR.3.1.46 – volume: 264 start-page: 11200 issn: 0021-9258 issue: 19 year: 1989 ident: 22 doi: 10.1016/S0021-9258(18)60449-1 – ident: 12 doi: 10.1159/000007174 – ident: 4 doi: 10.1038/nbt1267 – ident: 6 doi: 10.1002/psc.704 |
| SSID | ssj0029081 |
| Score | 1.7677027 |
| Snippet | Cationic and amphiphilic antimicrobial peptides (AMPs) such as α-defensins and cathelicidins are factors related to innate immunity. In the present study, we... Cationic and amphiphilic antimicrobial peptides (AMPs) such as alpha -defensins and cathelicidins are factors related to innate immunity. In the present study,... |
| SourceID | proquest crossref jstage |
| SourceType | Aggregation Database Publisher |
| StartPage | 618 |
| SubjectTerms | antimicrobial peptide Enterococcus faecalis human α-defensin innate immunity |
| Title | Defensive Effects of Human Antimicrobial Peptide α-Defensins against Enterococcus faecalis |
| URI | https://www.jstage.jst.go.jp/article/jhs/56/5/56_5_618/_article/-char/en https://search.proquest.com/docview/869570604 |
| Volume | 56 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | Journal of Health Science, 2010, Vol.56(5), pp.618-622 |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3db9MwELfY4AEJIT5F-ZIleIs80sZ27MeJZhrqNpCWSXuLHMcZHVqCSPtQ_nrOH0laTQh44MWtXMeq7n4-313uA6H3zExlWhpKWCUMoVqWRPF0SkopKLU6fpzaROHj8_TsUswzmo0O_XHuv3Ia5oDXNnP2H7g9bAoT8B14DiNwHca_4vvc1CEmPRtDNbyr_rBZLW-WrvISMOaLjWepTOSybSMSnmu6SF0p-FhFLl6gBYGp111UK6NtrcTfKLM-nTLqk4RAbc3bbxv_Vgeu42bL4XC63LSdayUcnZPBZ7toN-pG7fhdT5c_164x-BjIvbCtn2yJ6dGPGzwWIWrVi9eEUiLTeEf--sLiAWdsS5jyIJn9vcx9_vItkT-jNo3h-mt3wPjB8Mh2Xe2zz8XRxclJkWeX-R66OwORZHtdzD8tBtNcxsKb5uH_hUK2sPeHcecd1eXeNWjvV7evcKeX5I_Qw8ADfOiR8BjdMc0T9MB7Y7FPMnuKigEVOKACtzV2qMA7qMABFdihAo-owAEVeBsVuEfFM3RxlOUfj0lorUE0i2crIsGSrLjUsQH7W2ld8VjrWJU6SWipuJ7BMZ0mQmvG04orxoUUTjUUdaIkqKTP0X7TNuYFwqqyZq1hpaw1rZkSNdjsYAwZlSQpq80EveupVnz3FVQKa3kCbQugbcF4AbSdIOEJOqwJJ6hfw_wAS4dfbF4iHPkJwj0LCpCN9oWXaky77grBJXPVoV7-eckrdN8Hhljv2mu0v_qxNm_QXlet3zqo_AIKj4XY |
| link.rule.ids | 315,782,786,4028,27932,27933,27934 |
| linkProvider | Flying Publisher |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Defensive+Effects+of+Human+Antimicrobial+Peptide+alpha+-Defensins+against+Enterococcus+faecalis&rft.jtitle=Journal+of+health+science+%28Tokyo%2C+Japan%29&rft.au=Miyoshi%2C+S-I&rft.au=Koyama%2C+K&rft.au=Mizuno%2C+T&rft.au=Kashihara%2C+M&rft.date=2010&rft.issn=1344-9702&rft.volume=56&rft.issue=5&rft.spage=618&rft.epage=622&rft_id=info:doi/10.1248%2Fjhs.56.618&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1344-9702&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1344-9702&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1344-9702&client=summon |